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An aptasensor based on the microscopic enumeration of encoding gold nanoparticles for the detection of C-reactive protein

机译：一种基于编码金纳米粒子的微观枚举进行C-反应蛋白的显微序的Aptasensor

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C-reactive protein (CRP) is a crucial clinical biomarker for inflammatory and cardiovascular diseases. Therefore, the sensitive, selective and convenient detection of CRP is of great significance. Using gold nanoparticles (AuNPs) and combining the specific interaction between an aptamer and CRP, we developed a simple and convenient assay for CRP detection. The aptamer-based probe was fabricated through the hybridization of CRP-aptamer immobilized on magnetic beads (MBs) to a short complementary DNA (cDNA) chain attached to AuNPs to form a MB-Aptamer-AuNP sandwich structure. Upon the addition of CRP, aptamer-cDNA dehybridization occurred due to the strong interaction between CRP and the aptamer, resulting in the release of AuNPs, which were subjected to DFM imaging and subsequently counted using the MATLAB program. The number of AuNPs was therefore positively correlated to the concentration of CRP and a detection limit as low as 2.71 nM was achieved. The current approach could also exclude the disturbance of other proteins, including thrombin, IgG, Lys and BSA. In addition, the concentration of CRP detected was in good agreement with the amount cast in bovine and mouse serum, indicating that the proposed probe is robust and accurate, and it is very promising for practical applications where CRP detection is necessary. The current strategy is also promising for the detection of other proteins where a suitable aptamer is selected.
机译：C-反应蛋白（CRP）是炎症和心血管疾病的关键临床生物标志物。因此，CRP的敏感，选择性和方便的检测具有重要意义。使用金纳米颗粒（AUNP）并结合适体和CRP之间的特异性相互作用，我们开发了用于CRP检测的简单方便的测定。通过固定在磁珠（MBS）上固定在磁珠（MBS）上的CRP-Aptamer的杂交来制造基于Aptamer的探针，以形成AUNP的短互补DNA（cDNA）链，以形成MB-Aptamer-AUNP夹层结构。在添加CRP时，由于CRP和适体之间的强相互作用而发生Aptamer-cDNA脱次化，导致剖腹产的释放，其进行DFM成像并随后使用MATLAB程序计算。因此，剖腹脉冲数与CRP的浓度正相关，并且达到2.71nm的检测极限。目前的方法还可以排除其他蛋白质的干扰，包括凝血酶，IgG，Lys和BSA。此外，检测到的CRP的浓度与牛和小鼠血清中的量吻合良好，表明所提出的探针是坚固且准确的，并且对于必要的CRP检测的实际应用非常有前途。目前的策略也有望检测选择合适的适体的其他蛋白质。

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    《RSC Advances》 |2019年第59期|共6页
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  • 正文语种 eng
  • 中图分类 化学;
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