Graphene oxide decorated with zinc oxide nanoflower, silver and titanium dioxide nanoparticles: fabrication, characterization, DNA interaction, and antibacterial activity

El-Shafai Nagi; El-Khouly Mohamed E.; El-Kemary Maged; Ramadan Mohamed; Eldesoukey Ibrahim; Masoud Mamdouh

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Graphene oxide decorated with zinc oxide nanoflower, silver and titanium dioxide nanoparticles: fabrication, characterization, DNA interaction, and antibacterial activity

机译：用氧化锌纳米辊，银和二氧化钛纳米粒子装饰的石墨烯氧化物：制造，表征，DNA相互作用和抗菌活性

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The fabrication, characterization, and antibacterial activity of novel nanocomposites based on graphene oxide (GO) nanosheets decorated with silver, titanium dioxide nanoparticles, and zinc oxide nanoflowers were examined. The fabricated nanocomposites were characterized by various techniques including X-ray diffraction, ultraviolet-visible light absorption and fluorescence spectroscopy, Brunauer-Emmett-Teller theory analysis, Fourier transform infrared, and scanning electron microscopy. The antibacterial activity of the GO-metal oxide nanocomposites against two Gram-positive and two Gram-negative bacteria was examined by using the standard counting plate methodology. The results showed that the fabricated nanocomposites on the surface of GO could inhibit the growth of microbial adhered cells, and consequently prevent the process of biofilm formation in food packaging and medical devices. To confirm the antibacterial activity of the examined GO-nanocomposites, we examined their interactions with bovine serum albumin (BSA) and circulating tumor DNA (ctDNA) by steady-state fluorescence spectroscopy. Upon addition of different amounts of fabricated GO-nanocomposites, the fluorescence intensities of the singlet states of BSA and ctDNA were considerably quenched. The higher quenching was observed in the case of GO-Ag-TiO2@ZnO nanocomposite compared with other control composites.

机译：基于石墨烯氧化物（GO）纳米晶片的新型纳米复合材料的制备，表征和抗菌活性进行了检查用银，二氧化钛纳米粒子和氧化锌氧化锌掺杂者装饰。通过包括X射线衍射，紫外线可见光吸收和荧光光谱，Brunauer-Emmett-Teller理论分析，傅里叶变换红外和扫描电子显微镜，其特征在于，所述制造的纳米复合材料的特征在于。通过使用标准计数板方法，检查储备氧化物纳米复合材料的抗菌活性，并使用标准计数板方法检查。结果表明，GOS表面上的制造纳米复合材料可抑制微生物粘附细胞的生长，从而防止食品包装和医疗装置中生物膜形成的过程。为了确认检测到的持续型去纳米复合材料的抗菌活性，通过稳态荧光光谱检查它们与牛血清白蛋白（BSA）和循环肿瘤DNA（CTDNA）的相互作用。在添加不同量的制造的去纳米复合材料后，BSA和CTDNA的单态态的荧光强度显着淬火。与其他对照复合材料相比，在Go-Ag-TiO 2 @ ZnO纳米复合材料的情况下观察到较高的猝灭。

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《RSC Advances》 |2019年第7期|共11页
作者
El-Shafai Nagi; El-Khouly Mohamed E.; El-Kemary Maged; Ramadan Mohamed; Eldesoukey Ibrahim; Masoud Mamdouh;
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Alexandria Univ Dept Chem Fac Sci Alexandria Egypt;

Kafrelsheikh Univ Dept Chem Fac Sci Kafr Al Sheikh Egypt;

Kafrelsheikh Univ Inst Nanosci &

Nanotechnol Kafr Al Sheikh Egypt;

Alexandria Univ Dept Chem Fac Sci Alexandria Egypt;

Kafrelsheikh Univ Dept Bacteriol Mycol &

Immunol Fac Vet Med Kafr Al Sheikh Egypt;

Alexandria Univ Dept Chem Fac Sci Alexandria Egypt;

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正文语种 eng
中图分类化学;
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1. Graphene oxide decorated with zinc oxide nanoflower, silver and titanium dioxide nanoparticles: fabrication, characterization, DNA interaction, and antibacterial activity [J] . El-Shafai Nagi, El-Khouly Mohamed E., El-Kemary Maged, RSC Advances . 2019,第7期

机译：用氧化锌纳米辊，银和二氧化钛纳米粒子装饰的石墨烯氧化物：制造，表征，DNA相互作用和抗菌活性
2. Fabrication and antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus of silver nanoparticle decorated reduced graphene oxide nanocomposites [J] . Nguyen Minh Dat, Vu Ngoc Phuong Linh, Le Anh Huy, Materials Technology . 2019,第7a8期

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Graphene oxide decorated with zinc oxide nanoflower, silver and titanium dioxide nanoparticles: fabrication, characterization, DNA interaction, and antibacterial activity

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