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Fabrication of an immunosensor for quantitative detection of breast cancer biomarker UBE2C

机译：用于定量检测乳腺癌生物标志物UBE2C的免疫传感器的制备

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摘要

This work reports the design of a new electrochemical impedimetric immunosensor for the direct determination of ubiquitin-conjugating enzymes 2C (UBE2C), a potential diagnostic biomarker for breast cancer. The immunosensor was fabricated by immobilizing the capture anti-UBE2C antibody onto a polyaniline (PANI) modified glassy carbon electrode (GCE) through glutaraldehyde crosslinking. The assembly process of the immunosensor was examined using scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The fabricated immunosensor enabled the detection of recombinant human UBE2C in the range of 500 pg mL -1 to 5 mg mL -1. The limit of detection and limit of quantification were found to be 7.907 pg mL -1 and 26.356 pg mL -1, respectively. The diagnostic application of the fabricated immunosensor was explored for the analysis of breast cancer cell line MCF-7 cell extract. The immunosensor demonstrated high selectivity for UBE2C. The fabricated immunosensor also exhibited good reproducibility and storage stability.
机译：这项工作报告了一种新的电化学阻抗免疫传感器，用于直接测定遍在蛋白缀合酶2C（UBE2C），乳腺癌潜在的诊断生物标志物。通过将捕获抗UBE2C抗体固定到聚苯胺（PANI）改性玻璃电极（GCE）通过戊二醛交联来制造免疫传感器。使用扫描电子显微镜，循环伏安法和电化学阻抗光谱检查免疫传感器的组装过程。制造的免疫传感器使重组人UBE2C的检测在500pg ml -1至5mg ml -1的范围内。发现检测极限和定量限制分别为7.907pg ml -1和26.356pg ml -1。探讨了制造的免疫传感器的诊断应用，用于分析乳腺癌细胞系MCF-7细胞提取物。免疫传感器对UBE2C表现出高选择性。制造的免疫传感器也表现出良好的再现性和储存稳定性。

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  • 来源
    《RSC Advances》 |2019年第29期|共8页
  • 作者

    Jayanthi V. S. P. K. Sankara Aditya; Das Asim Bikas; Saxena Urmila;

  • 作者单位

    Natl Inst Technol Warangal Dept Biotechnol Warangal 506004 Telangana India;

    Natl Inst Technol Warangal Dept Biotechnol Warangal 506004 Telangana India;

    Natl Inst Technol Warangal Dept Biotechnol Warangal 506004 Telangana India;

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  • 正文语种 eng
  • 中图分类 化学;
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