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首页> 外文期刊>RSC Advances >Direct extraction of astaxanthin from the microalgae Haematococcus pluvialis using liquid-liquid chromatography
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Direct extraction of astaxanthin from the microalgae Haematococcus pluvialis using liquid-liquid chromatography

机译：使用液 - 液相色谱法从微藻血清藻普（Pluvialis）直接提取虾青蛋白

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摘要

The microalgae Haematococcus pluvialis (H. pluvialis) is used for biotechnological production of the red carotenoid astaxanthin. Astaxanthin synthesis involves the formation of a rigid cell wall that impedes direct astaxanthin extraction into a solvent. During the subsequent downstream processing, the algal broth is harvested by centrifugation, dried and mechanically disrupted; finally, astaxanthin is extracted with supercritical CO2. In this study, an alternative extraction process was established, using a liquid-liquid chromatographic column to directly extract astaxanthin from the fermentation broth into a solvent. To achieve this, germination of H. pluvialis cyst cells was initiated, resulting in the release of flagellated zoospores into the fermentation broth. It was shown that astaxanthin could be extracted from the zoospores directly from the algal broth using different solvents; with ethyl acetate, yields reaching 85% were achieved in a shake-flask extraction. Using a liquid-liquid chromatographic column, astaxanthin concentrations reaching 500 mg L-1 were obtained, corresponding to eightfold concentration of the astaxanthin content in the fermentation broth. The mechanical cell disruption, drying and extraction with supercritical CO2 in the conventional astaxanthin production can be replaced by a direct astaxanthin extraction process, using a liquid-liquid chromatographic column. This allows direct astaxanthin extraction at the site of H. pluvialis production.
机译：Microalgae Haematococcus Pluvialis（H pluvialis）用于红色类胡萝卜素虾的生物技术生产。虾青素合成涉及形成刚性细胞壁，其将直接虾青素提取到溶剂中。在随后的下游加工过程中，通过离心收获藻类肉汤，干燥并机械破坏;最后，用超临界CO 2提取虾青蛋白。在该研究中，使用液 - 液相色谱柱建立替代提取过程，将虾青蛋白从发酵液中直接提取到溶剂中。为了实现这一点，开始发芽HPluvialis囊肿细胞，导致鞭毛化动物孢子释放到发酵液中。结果表明，使用不同的溶剂可以直接从动物孢子中从动物孢子液中提取;乙酸乙酯，在摇瓶萃取中实现达到85％的产率。使用液体色谱柱，获得达到500mg L-1的虾青素浓度，对应于发酵液中的虾青素含量的八倍浓度。在常规的虾青蛋白产生中的超临界CO2中的机械细胞破坏，干燥和萃取可以通过使用液 - 液相色谱柱的直接虾青素萃取工艺代替。这允许在Pluvialis生产现场直接虾青素提取。

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  • 来源
    《RSC Advances》 |2019年第40期|共11页
  • 作者

    Bauer Andreas; Minceva Mirjana;

  • 作者单位

    Tech Univ Munich TUM Sch Life Sci Biothermodynam Maximus von Imhof Forum 2 D-85354 Freising Weihenstephan Germany;

    Tech Univ Munich TUM Sch Life Sci Biothermodynam Maximus von Imhof Forum 2 D-85354 Freising Weihenstephan Germany;

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  • 正文语种 eng
  • 中图分类 化学;
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