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Selection and preliminary application of a single stranded DNA aptamer targeting colorectal cancer serum

机译：单链DNA适体靶向结直肠癌血清的选择和初步应用

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Colorectal cancer is one of the common causes of malignant tumors in recent years, thus the discovery of potential compounds that detect the occurrence of colorectal cancer by efficient approaches is necessary. In this study, the method of systematic evolution of ligands by exponential enrichment (SELEX) was used for recognizing serum from colorectal cancer patients by a single-stranded DNA library of aptamers assisted by single-walled carbon nanotubes (SWCNTs) to remove single-stranded DNA with low affinity. Ten rounds of selection were applied using colorectal cancer serum as a target with the serum of healthy individuals as a control. As the result, we have successfully identified four candidate aptamers after high-throughput genome sequencing analysis, comparison analysis and secondary structure prediction. Among them, aptamer Seq-2 exhibited the highest affinity and the strongest selectivity with an equilibrium dissociation constant (K-d) of 11.31 +/- 3.25 nM and a C-t difference value of 4.25 +/- 0.38 between the colorectal cancer group and the healthy group. Moreover, with fifty negative control serum samples, the positive detection rate of fifty positive serum samples tested by aptamer Seq-2 was over 90%. In particular, aptamer Seq-2 can strongly bind the colorectal cancer serum, less strongly bind the non-colon cancer serum and hardly bind the healthy serum. Therefore, aptamer Seq-2 presents enormous potential for exploring as a tumor diagnostic kit and detecting unknown tumor markers in serum to reflect colorectal cancer.
机译：结肠直肠癌是近年来恶性肿瘤的常见原因之一，因此需要通过有效方法检测结直肠癌发生的潜在化合物的发现。在该研究中，通过指数富集（SELEX）的配体系统演化的方法用于通过单链碳纳米管（SWCNTs）的单链DNA文库来识别来自结直肠癌患者的血清，以除去单链具有低亲和力的DNA。使用结肠直肠癌血清作为对照作为对照的靶标作为靶向靶向靶。结果，在高通量基因组测序分析，比较分析和二级结构预测之后，我们已经成功地鉴定了四种候选适体。其中，适体SEQ-2表现出最高的亲和力，并用11.31 +/- 3.25 nM的平衡解离常数（Kd）和4.25 +/- 0.38的结直肠癌组和健康组之间的差异的Ct值最强的选择性。此外，含有五十个阴性对照血清样品，由Aptamer SEQ-2测试的50个阳性血清样品的阳性检测率超过90％。特别是，适体SEQ-2可以强烈地结合结肠直肠癌血清，较小强烈地结合非结肠癌血清，几乎不结合健康的血清。因此，Aptamer SEQ-2呈现为肿瘤诊断试剂盒和检测血清中未知的肿瘤标志物的巨大潜力，以反映结直肠癌。

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    《RSC Advances》 |2019年第66期|共10页
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  • 正文语种 eng
  • 中图分类 化学;
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