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Functionalization of polyacrylamide for nanotrapping positively charged biomolecules

机译：聚丙烯酰胺用于纳米型带正电荷的生物分子的官能化

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摘要

Engineering new materials which are capable of trapping biomolecules in nanoscale quantities, is crucial in order to achieve earlier diagnostics in different diseases. This article demonstrates that using free radical copolymerization, polyacrylamide can be successfully functionalized with specific synthons for nanotrapping positively charged molecules, such as numerous proteins, through electrostatic interactions due to their negative charge. Specifically, two functional random copolymers, acrylamide/acrylic acid (1) and acrylamide/acrylic acid/N-(pyridin-4-yl-methyl)acrylamide (2), whose negative net charges differ in their water solutions, were synthetized and their ability to trap positively charged proteins was studied using myoglobin as a proof-of-concept example. In aqueous solutions, copolymer 1, whose net charge for a 100 chain fragment (Q(pH 6)/M) is -1.323 x 10(-3), interacted with myoglobin forming a stable monodisperse nanosuspension. In contrast, copolymer 2, whose value of Q(pH 6)/M equals -0.361 x 10(-3), was not able to form stable particles with myoglobin. Nevertheless, thin films of both copolymers were grown using a dewetting process, which exhibited nanoscale cavities capable of trapping different amounts of myoglobin, as demonstrated by bimodal AFM imaging. The simple procedures used to build protein traps make this engineering approach promising for the development of new materials for biomedical applications where trapping biomolecules is required.
机译：能够在纳米级数量中捕获生物分子的工程新材料是至关重要的，以实现不同疾病的早期诊断。本文表明，使用自由基共聚，聚丙烯酰胺可以通过针对纳米发出的带正电荷的分子（例如许多蛋白质）成功官能化的聚丙烯酰胺通过静电相互作用而成功官能化。具体地，两种功能性随机共聚物，丙烯酰胺/丙烯酸（1）和丙烯酰胺/丙烯酸/ N-（吡啶-4-基 - 甲基）丙烯酰胺（2），其负净电荷在其水溶液中不同，并合成及其使用Myoglobin作为概念验证示例，研究了捕获带正电荷蛋白质的能力。在水溶液中，为100个链碎片的净电荷（Q（pH6）/ m）为-1.323×10（-3），与肌红蛋白相互作用，形成稳定的单分散性纳米柱术。相反，共聚物2，其值Q（pH6）/ m等于-0.361×10（-3），不能与肌红蛋白形成稳定的颗粒。然而，使用脱水工艺生长两种共聚物的薄膜，其显示能够捕获不同量的肌红蛋白的纳米级腔，如双峰AFM成像所证明的那样。用于构建蛋白质陷阱的简单程序使得这种工程方法对需要进行需要的生物医学应用的新材料的开发，这是需要捕获生物分子的新材料。

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  • 来源
    《RSC Advances》 |2019年第27期|共8页
  • 作者

    Davydova Nadejda; Rodriguez Xavier R.; Blazquez Carlos; Gomez Andres; Perevyazko Igor; Guasch Judith; Sergeev Vladimir; Laukhina Elena; Ratera Imma; Veciana Jaume;

  • 作者单位

    Russian Acad Sci AN Nesmeyanov Inst Organoelement Cpds Vavilova St 28 Moscow 119991 Russia;

    CIBER BBN Campus UAB Barcelona 08193 Spain;

    CIBER BBN Campus UAB Barcelona 08193 Spain;

    Inst Mat Sci Barcelona ICMAB CSIC SPM Serv Campus UAB Barcelona 08193 Spain;

    St Petersburg State Univ Dept Mol Biophys &

    Polymer Phys Ulyanovskaya St 1 St Petersburg 198504 Russia;

    CIBER BBN Campus UAB Barcelona 08193 Spain;

    Russian Acad Sci AN Nesmeyanov Inst Organoelement Cpds Vavilova St 28 Moscow 119991 Russia;

    CIBER BBN Campus UAB Barcelona 08193 Spain;

    CIBER BBN Campus UAB Barcelona 08193 Spain;

    CIBER BBN Campus UAB Barcelona 08193 Spain;

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  • 中图分类 化学;
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