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首页> 外文期刊>RSC Advances >Tannic acid-stabilized gold nano-particles are superior to native tannic acid in inducing ROS-dependent mitochondrial apoptosis in colorectal carcinoma cells via the p53/AKT axis
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Tannic acid-stabilized gold nano-particles are superior to native tannic acid in inducing ROS-dependent mitochondrial apoptosis in colorectal carcinoma cells via the p53/AKT axis

机译：鞣酸稳定的金纳米颗粒优于天然单宁酸，在通过P53 / AKT轴诱导结直肠癌细胞中的ROS依赖性线粒体细胞凋亡

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摘要

Gold nanoparticle formulated tannic acid (AuNP-TA) was synthesized, and its anticancer activity was compared to that of free tannic acid (TA). The half maximal inhibitory concentration (IC50) was reduced by half when cell lines were treated with AuNP-TA as compared to IC50 values upon free TA treatment. Both showed better cytotoxic activity in HCT116 cell line as compared to MCF7 and HepG2. AuNP-TA induced death of HCT116 cells was associated with characteristic apoptotic changes. At the same treatment dose, AuNP-TA generated more ROS, caused a more extensive DNA damage and promoted higher expression of p53 and p21 than TA. Treatment with AuNP-TA regulated generation of p53 and ROS bi-directionally. Binding studies showed that TA lowered the expression of Akt, which inhibited the survival of colon cancer cells. Also, cell cycle arrest at the G2/M phase, enhanced expression of caspase-3/9, Bak, and Bax, loss of mitochondrial membrane potential, and enhanced level of cytosolic cytochrome c were observed in AuNP-TA treated HCT116 cells. Thus, AuNP-TA is more efficient than TA in inducing apoptotic cell death of HCT116 cells via the ROS/P53/Akt axis.
机译：合成金纳米粒子制定的单宁赤烷酸（AUNP-TA），将其抗癌活性与游离单宁酸（TA）进行比较。当与AUNP-TA处理相比，与IC 50值相比，在游离TA处理上与IC 50值相比，半最大抑制浓度（IC50）减少了一半。与MCF7和HepG2相比，两者在HCT116细胞系中显示出更好的细胞毒性活性。 AUNP-TA诱导HCT116细胞的死亡与特征凋亡变化有关。在相同的治疗剂量，AUNP-TA产生更多ROS，导致更广泛的DNA损伤并促进P53和P21的表达比TA更高。用AUNP-TA调节P53和ROS双向治疗。结合研究表明，TA降低了AKT的表达，抑制结肠癌细胞的存活。此外，在AUNP-TA处理的HCT116细胞中观察到Caspase-3/9，Bak和Bax的增强表达，Bak和Bax，线粒体膜电位的丧失和增强水平的细胞周期停滞，对线粒体膜电位的增强水平，并且在细胞溶质细胞色素C的增强水平。因此，通过ROS / P53 / AKT轴诱导HCT116细胞的凋亡细胞死亡，AUNP-TA更有效。

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  • 来源
    《RSC Advances》 |2019年第14期|共14页
  • 作者

    Nag Sayoni; Manna Krishnendu; Saha Krishna Das;

  • 作者单位

    Indian Inst Chem Biol CSIR Canc Biol &

    Inflammatory Disorder Div 4 Raja SC Mullick Rd Kolkata 700032 W Bengal India;

    Indian Inst Chem Biol CSIR Canc Biol &

    Inflammatory Disorder Div 4 Raja SC Mullick Rd Kolkata 700032 W Bengal India;

    Indian Inst Chem Biol CSIR Canc Biol &

    Inflammatory Disorder Div 4 Raja SC Mullick Rd Kolkata 700032 W Bengal India;

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  • 正文语种 eng
  • 中图分类 化学;
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