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Importance of the biofilm matrix for the erosion stability of Bacillus subtilis NCIB 3610 biofilms

机译：生物膜基质对枯草芽孢杆菌的侵蚀稳定性的重要性，枯草芽孢杆菌Ncib 3610生物膜

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摘要

Production and secretion of biomolecules can provide new emergent functionalities to the synthesizing organism. In particular, the secretion of extracellular polymeric substances (EPS) by biofilm forming bacteria creates a biofilm matrix that protects the individual bacteria within the biofilm from external stressors such as antibiotics, chemicals and shear flow. Although the main matrix components of biofilms formed by Bacillus subtilis are known, it remains unclear how these matrix components contribute to the erosion stability of B. subtilis biofilms. Here, we combine different biophysical techniques to assess this relation. In particular, we quantify the importance of specific biofilm matrix components on the erosion behavior of biofilms formed by the well-studied Bacillus subtilis NCIB 3610. We find that the absence of biofilm matrix components decreases the erosion stability of NCIB 3610 biofilms in water, largely by abolishing the hydrophobic surface properties of the biofilm and by reducing the biofilm stiffness. However, the erosion resistance of NCIB 3610 biofilms is strongly increased in the presence of metal ions or the antibiotic ciprofloxacin. In the first case, unspecific ionic cross-linking of biofilm components or individual bacteria seems to be responsible for the observed effect, and in the second case there seems to be an unspecific interaction between the antibiotic and the biofilm matrix. Taken together, our results emphasize the importance of the biofilm matrix to reduce biofilm erosion and give insights into how the specific biomolecules interact with certain chemicals to fulfill this task.
机译：生物分子的生产和分泌可以为合成生物提供新的紧急功能。特别地，Biofilm成形细菌细胞外聚合物物质（EPS）的分泌产生生物膜基质，可保护生物膜内的个体细菌免受抗生素，化学品和剪切流动的外部压力源。尽管通过枯草芽孢杆菌形成的生物膜的主要基质组分是已知的，但仍然不明确于这些基质组分有助于B.枯草芽孢杆菌生物膜的侵蚀稳定性。在这里，我们结合了不同的生物物理技术来评估这一关系。特别是，我们量化了特定生物膜基质组分对由学习的枯草芽孢杆菌Ncib 3610形成的生物膜的侵蚀行为的重要性。我们发现没有生物膜基质组分的缺失降低了Ncib 3610生物膜在水中的侵蚀稳定性，主要是通过废除生物膜的疏水性表面性质，并通过减少生物膜刚度。然而，在金属离子或抗生素环丙沙星的存在下，NCIB 3610生物膜的侵蚀抗性强烈增加。在第一种情况下，生物膜组分或单个细菌的非特异性离子交联似乎对观察到的效果负责，并且在第二种情况下似乎存在抗生素和生物膜基质之间的非特异性相互作用。我们的结果携带，我们的结果强调了生物膜基质的重要性，以减少生物膜侵蚀，并向特定生物分子与某些化学品相互作用以实现这项任务的洞察。

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  • 来源
    《RSC Advances》 |2019年第20期|共9页
  • 作者

    Klotz M.; Kretschmer M.; Goetz A.; Ezendam S.; Lieleg O.; Opitz M.;

  • 作者单位

    Ludwig Maximilians Univ Munchen Ctr NanoSci Fac Phys Munich Germany;

    Tech Univ Munich Munich Sch BioEngn Garching Germany;

    Ludwig Maximilians Univ Munchen Ctr NanoSci Fac Phys Munich Germany;

    Ludwig Maximilians Univ Munchen Ctr NanoSci Fac Phys Munich Germany;

    Tech Univ Munich Munich Sch BioEngn Garching Germany;

    Ludwig Maximilians Univ Munchen Ctr NanoSci Fac Phys Munich Germany;

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  • 正文语种 eng
  • 中图分类 化学;
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