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Kinetic and spectroscopic responses of pH-sensitive nanoparticles: influence of the silica matrix

机译：pH敏感纳米粒子的动力学和光谱响应：二氧化硅基质的影响

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Intracellular pH sensing with fluorescent nanoparticles is an emerging topic as pH plays several roles in physiology and pathologic processes. Here, nanoparticle-sized pH sensors (diameter far below 50 nm) for fluorescence imaging have been described. Consequently, a fluorescent derivative of pH-sensitive hydroxypyrene with pK(a) = 6.1 was synthesized and subsequently embedded in core and core-shell silica nanoparticles via a modified Stober process. The detailed fluorescence spectroscopic characterization of the produced nanoparticles was carried out for retrieving information about the environment within the nanoparticle core. Several steady-state and time-resolved fluorescence spectroscopic methods hint to the screening of the probe molecule from the solvent, but it sustained interactions with hydrogen bonds similar to that of water. The incorporation of the indicator dye in the water-rich silica matrix neither changes the acidity constant nor dramatically slows down the protonation kinetics. However, cladding by another SiO2 shell leads to the partial substitution of water and decelerating the response of the probe molecule toward pH. The sensor is capable of monitoring pH changes in a physiological range by using ratiometric fluorescence excitation with lambda(ex) = 405 nm and lambda(ex) = 488 nm, as confirmed by the confocal fluorescence imaging of intracellular nanoparticle uptake.
机译：用荧光纳米粒子的细胞内pH感测是一种新兴的话题，因为pH在生理学和病理过程中发挥着几个作用。这里，已经描述了纳米粒子大小的pH传感器（直径远低于50nm），用于荧光成像。因此，合成了具有PK（a）= 6.1的pH敏感羟基丙烯的荧光衍生物，并随后通过改性的阶级方法嵌入核心和核 - 壳二氧化硅纳米粒子中。进行了所生产的纳米颗粒的详细荧光光谱表征，用于检索纳米颗粒核心内的环境的信息。几个稳态和时间分辨的荧光光谱方法提示从溶剂中筛选探针分子，但它与类似于水的氢键相互作用。在水性富含硅基基质中的指示剂染料掺入酸度常数也不会显着减缓质子化动力学。然而，另一个SiO2壳的包层导致水的部分取代和减速探针分子朝向pH的响应。通过使用细胞内纳米颗粒摄取的共聚焦荧光成像证实，通过使用λ（ex）= 405nm和Lambda（前）= 488nm的比率荧光激发来监测生理范围的pH变化。

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    《RSC Advances》 |2019年第61期|共11页
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  • 正文语种 eng
  • 中图分类 化学;
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