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PEGylation of protein-imprinted nanocomposites sandwiching CdTe quantum dots with enhanced fluorescence sensing selectivity

机译：蛋白质印迹纳米复合材料的聚乙二醇化夹在CDTE量子点具有增强的荧光感测选择性

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Fluorescent sensors combining the selective recognition of protein molecularly imprinted polymers (MIPs) and the fluorescent sensing of quantum dots (QDs) have been studied considerably, but their fluorescence sensing selectivity for the target proteins remains to be increased. Herein, we propose a strategy for increasing the sensing selectivity by post-imprinting PEGylation of surface protein-imprinted nanocomposites with embedded QDs. With bovine hemoglobin (BHb) as a model protein template, protein MIP nanolayers were anchored over the CdTe QD decorated SiO2 nanoparticles by the sol-gel process using aminopropyltriethoxy silane and tetraethoxysilicane. PEG chains were then grafted onto the surface of the imprinted nanostructures via the nucleophilic reaction of the surface amine groups with N-hydroxysuccinimide ester-terminal methoxy-PEG, followed by template removal. The resultant PEGylated sensors showed significantly improved aqueous dispersion stability compared with the non-PEGylated controls. More importantly, such PEGylation greatly increased the fluorescence response selectivity, with the Stern-Volmer equation based imprinting factor increasing from 2.7 to 5.4. The PEGylated sensors were applied to determine BHb in bovine serum samples with satisfactory recoveries at three spiking levels ranging from 94.3 to 103.7%, indicating their potential application in real samples.
机译：已经大大研究了组合蛋白质印迹聚合物（MIPS）的选择性识别的荧光传感器和量子点（QDS）的荧光感测，但它们对靶蛋白的荧光感测选择性仍有待增加。在此，我们提出了一种通过用嵌入的QDS的表面蛋白质印迹纳米复合材料的后印刷的PEG化来提高感测选择性的策略。用牛血红蛋白（BHB）作为模型蛋白质模板，通过使用氨基丙基三乙氧基硅烷和四乙氧基硅烷通过溶胶 - 凝胶工艺锚定蛋白质MIP纳米纳米通过溶胶 - 凝胶工艺锚定。然后通过用N-羟基琥珀酰亚胺酯 - 甲氧基-PEG的表面胺基团的亲核反应将PEG链接枝到印迹纳米结构的表面上，然后用模板去除。与非聚乙二醇化对照相比，所得聚乙二醇化的传感器显示出显着改善的水分散稳定性。更重要的是，这种PEG化大大增加了荧光响应选择性，基于船尾的Volmer方程基于2.7至5.4的压印因子增加。施用聚乙二醇化的传感器以确定牛血清样品中的BHB，其三个尖峰水平的令人满意的回收率范围为94.3至103.7％，表明它们在真实样品中的潜在应用。

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    《RSC Advances》 |2019年第65期|共9页
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  • 正文语种 eng
  • 中图分类 化学;
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