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首页> 外文期刊>RSC Advances >A glucose biosensor based on the immobilization of glucose oxidase and Au nanocomposites with polynorepinephrine
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A glucose biosensor based on the immobilization of glucose oxidase and Au nanocomposites with polynorepinephrine

机译：一种基于葡萄糖氧化酶和Au纳米复合材料的葡萄糖生物传感器与多诺啡碱

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摘要

A glucose biosensor based on Au nanoparticles (AuNPs), glucose oxidase (GOD) and polynorepinephrine (PNE) was fabricated by a simple and green approach. PNE-functionalized AuNPs (AuNPs@ PNE) were synthetized by the polymerization of norepinephrine (NE) onto the surfaces of AuNPs and casted on an Au electrode. After dropping a solution containing NE and GOD on the AuNPs@ PNE-modified Au electrode and oxidizing the monomer NE by cyclic voltammetry, a PNE, GOD and AuNP-modified Au electrode (PNE/GOD/AuNPs@ PNE/Au) was obtained. The biosensor presented high sensitivity of 35.4 mA mM 1 cm 2 to glucose in the range from 0.003 mM to 3.43 mM with a response time of less than 3 s, a detection limit of 1.34 mM at a signal/noise ratio of 3, a low Michaelis-Menten constant (6.8 mM) and outstanding selectivity as well as stability. Moreover, the sensitivity and linear detection range of the asprepared biosensor were further enhanced by the addition of the redox mediator p-benzoquinone.
机译：通过简单和绿色的方法制造基于Au纳米颗粒（AUNP），葡萄糖氧化酶（GNNE），葡萄糖氧化酶（PNE）的葡萄糖生物传感器。 通过将Norepinephrine（Ne）的聚合在AUNP的表面上并浇铸在Au电极上并浇铸合成PNE官能化AUNPS（AUNPS @ PNE）。 丢弃含有NE和上帝的溶液在AUNPS @ PNE-改性的Au电极上并通过循环伏安法氧化单体NE，获得PNE，上帝和AUNP改性的AU电极（PNE / GOD / AUNPS @ PNE / AU）。 生物传感器的高灵敏度为35.4 mm 1cm 2的葡萄糖，响应时间为0.003mm至3.43mm，响应时间小于3 s，检测限为1.34 mm，信号/噪声比为3，低 Michaelis-Menten恒定（6.8毫米）和出色的选择性以及稳定性。 此外，通过添加氧化还原介体对苯醌进一步增强了asprepared生物传感器的灵敏度和线性检测范围。

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  • 来源
    《RSC Advances》 |2019年第29期|共8页
  • 作者

    Liu Yang; Nan Xu; Shi Wei; Liu Xin; He Zi; Sun Yanan; Ge Dongtao;

  • 作者单位

    Xiamen Univ Key Lab Biomed Engn Fujian Prov Univ Res Ctr Biomed Engn Xiamen Dept Biomat Coll Mat Xiamen 361005 Fujian Peoples R China;

    Xiamen Univ Key Lab Biomed Engn Fujian Prov Univ Res Ctr Biomed Engn Xiamen Dept Biomat Coll Mat Xiamen 361005 Fujian Peoples R China;

    Xiamen Univ Key Lab Biomed Engn Fujian Prov Univ Res Ctr Biomed Engn Xiamen Dept Biomat Coll Mat Xiamen 361005 Fujian Peoples R China;

    Xiamen Univ Key Lab Biomed Engn Fujian Prov Univ Res Ctr Biomed Engn Xiamen Dept Biomat Coll Mat Xiamen 361005 Fujian Peoples R China;

    Xiamen Univ Key Lab Biomed Engn Fujian Prov Univ Res Ctr Biomed Engn Xiamen Dept Biomat Coll Mat Xiamen 361005 Fujian Peoples R China;

    Xiamen Univ Key Lab Biomed Engn Fujian Prov Univ Res Ctr Biomed Engn Xiamen Dept Biomat Coll Mat Xiamen 361005 Fujian Peoples R China;

    Xiamen Univ Key Lab Biomed Engn Fujian Prov Univ Res Ctr Biomed Engn Xiamen Dept Biomat Coll Mat Xiamen 361005 Fujian Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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