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首页> 外文期刊>RSC Advances >A UPLC-MS/MS application for comparisons of the hepatotoxicity of raw and processed Xanthii Fructus by energy metabolites
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A UPLC-MS/MS application for comparisons of the hepatotoxicity of raw and processed Xanthii Fructus by energy metabolites

机译：UPLC-MS / MS应用，用于通过能量代谢物进行原料和加工XONTHII果实的肝毒性比较

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摘要

The ripe fruit of Xanthium strumarium L. (Xanthii Fructus) cannot be widely used as a Chinese herbal medicine (CHM) owing to its hepatotoxicity. However, Xanthii Fructus (XF) can be used effectively and safely after correct processing based on traditional experience, although a high hepatotoxicity risk remains owing to improper usage. Therefore, the processing methods used must be clarified to ensure safety. The adenosine-5'-triphosphate (ATP) level in tissues is an important indicator reflecting the functional status of liver cells. Therefore, this study aims to evaluate the hepatotoxicity of XF using UPLC-MS/MS. The hepatotoxicity of raw XF (RXF) and XF processed by intermediary energy metabolites (PXF) is compared. The method is evaluated for its analytical performance and successfully applied to the quantification of ATP, adenosine-5'-diphosphate (ADP), adenosine-5'-monophosphate (AMP), atractyloside, and carboxyatractyloside in mouse liver. The hepatotoxicity results also indicate that the toxicity of XF is decreased after processing, perhaps due to the decrease in atractyloside and carboxyatractyloside contents. Importantly, the experimental evidence provides a rationale for the reduction in toxicity. These data show that mouse livers are damaged between the days 20 and 30 of RXF oral administration, and that the ATP level is decreased. Importantly, no significant difference is observed between the PXF treatment group and control group, while the RXF treatment group is significantly different. Therefore, processing can reduce the toxicity of XF.
机译：黄嘌呤纹状体L.（Xanthii Fructus）的成熟果实不能被广泛用作中国草药（CHM）由于其肝毒性。然而，XANTHII玻璃（XF）可以在基于传统经验的正确加工后有效和安全地使用，尽管仍然是使用不当的肝毒性风险。因此，必须澄清所使用的处理方法以确保安全性。组织中的腺苷-5'-三磷酸（ATP）水平是反映肝细胞功能状态的重要指标。因此，本研究旨在使用UPLC-MS / MS评估XF的肝毒性。比较由中间能量代谢物（PXF）处理的原始XF（RXF）和XF的肝毒性。评价该方法的分析性能并成功地应用于ATP，腺苷-5'-二磷酸（ADP），腺苷-5'-单磷酸（AMP），亚替纳苷和羧酰脱酰基在小鼠肝脏中的定量。肝毒性结果还表明，在加工后XF的毒性降低，也许是由于亚静弧菌和羧酰脱酰含量的降低。重要的是，实验证据提供了减少毒性的理由。这些数据显示，鼠标肝脏在RXF口服管理的日期和30之间损坏，并且ATP水平降低。重要的是，PXF治疗组和对照组之间没有观察到显着差异，而RXF治疗组显着不同。因此，处理可以降低XF的毒性。

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  • 来源
    《RSC Advances》 |2019年第5期|共7页
  • 作者

    Jiang Hai; Yang Liu; Xing Xudong; Yan Meiling; Guo Xinyue; Hou Ajiao; Man Wenjing; Yang Bingyou; Wang Qiuhong; Kuang Haixue;

  • 作者单位

    Heilongjiang Univ Chinese Med Minist Educ Key Lab Chinese Mat Med Harbin 150040 Heilongjiang Peoples R China;

    Heilongjiang Univ Chinese Med Minist Educ Key Lab Chinese Mat Med Harbin 150040 Heilongjiang Peoples R China;

    Heilongjiang Univ Chinese Med Minist Educ Key Lab Chinese Mat Med Harbin 150040 Heilongjiang Peoples R China;

    Heilongjiang Univ Chinese Med Minist Educ Key Lab Chinese Mat Med Harbin 150040 Heilongjiang Peoples R China;

    Heilongjiang Univ Chinese Med Minist Educ Key Lab Chinese Mat Med Harbin 150040 Heilongjiang Peoples R China;

    Heilongjiang Univ Chinese Med Minist Educ Key Lab Chinese Mat Med Harbin 150040 Heilongjiang Peoples R China;

    Heilongjiang Univ Chinese Med Minist Educ Key Lab Chinese Mat Med Harbin 150040 Heilongjiang Peoples R China;

    Heilongjiang Univ Chinese Med Minist Educ Key Lab Chinese Mat Med Harbin 150040 Heilongjiang Peoples R China;

    Heilongjiang Univ Chinese Med Minist Educ Key Lab Chinese Mat Med Harbin 150040 Heilongjiang Peoples R China;

    Heilongjiang Univ Chinese Med Minist Educ Key Lab Chinese Mat Med Harbin 150040 Heilongjiang Peoples R China;

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