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Effects of putrescine on gene expression in relation to physical barriers and antioxidant capacity in organs of weaning piglets

机译：Putrescine对断奶仔猪物理障碍和抗氧化能力相关基因表达的影响

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摘要

Weaning stress can cause metabolic disorders, gastrointestinal dysfunction, physical barrier injury and disease susceptibility, thus leading to impaired growth and health of animals. Putrescine has the potential to reduce stress effects. However, the role of putrescine supplementation on barrier function and antioxidant capacity in animals' organs is largely unknown. This study evaluates the effects of putrescine on the physical barrier function, antioxidant status and related signalling molecule levels of weaning piglets' organs. A total of 24 weaning piglets were assigned to four treatment groups: (1) basal diet (control) and basal diets supplemented with (2) 0.05%, (3) 0.1% and (4) 0.15% putrescine. At the end of the 11 day experiment, ileum, liver, thymus and spleen samples were collected from the piglets. Compared with the control group, 0.15% putrescine can significantly increase anti-hydroxyl radical capacity (ileum and spleen), anti-superoxide anion capacity (liver, thymus and spleen), catalase (ileum, liver, thymus and spleen), total superoxide dismutase (ileum, thymus and spleen), glutathione peroxidase (ileum, liver and thymus), glutathione S-transferase activity (ileum, liver, thymus and spleen), glutathione content (liver and spleen) and total antioxidant capacity (ileum and thymus); decrease malondialdehyde (ileum, liver, thymus and spleen), protein carbonyl content (ileum, liver, thymus and spleen); enhance mRNA expression of zonula occludens (ZO)-1 (spleen), ZO-2 (liver, thymus and spleen), occludin (ileum, liver, thymus and spleen), claudin 1 (ileum, liver, thymus and spleen), claudin 2 (ileum, thymus and spleen), claudin 3 (ileum, liver, thymus and spleen), claudin 14 (ileum, liver and spleen), claudin 16 (ileum and liver), superoxide dismutase 1 (ileum, liver and thymus), glutathione peroxidase 1 (ileum, liver, thymus and spleen), catalase (ileum, liver, thymus and spleen), glutathione reductase (thymus and spleen), glutathione S-transferase (ileum, liver, thymus and spleen) and nuclear erythroid 2-related factor 2 (liver and thymus); decrease mRNA level of myosin light chain kinase (ileum, liver, thymus and spleen) and Kelch-like ECH-associated protein 1 (liver and spleen) (P < 0.05). 0.05% putrescine can significantly affect some of the above-mentioned parameters (P < 0.05). Collectively, putrescine supplementation improves organs' physical barrier function and antioxidant capacity in dose- and tissue-dependent and independent effects; such improvements are beneficial to the health of weaning piglets.
机译：断奶应激会导致代谢紊乱，胃肠功能障碍，物理障碍损伤和疾病易感性，从而导致动物的生长和健康受损。 Putrescine有可能降低应力效应。然而，Putrescine补充对动物器官的屏障功能和抗氧化能力的作用在很大程度上是未知的。本研究评估了Putrescine对断奶仔猪器官的物理屏障功能，抗氧化状态和相关信号分子水平的影响。共有24个断奶仔猪分配到四个治疗组：（1）基础饮食（对照）和补充有（2）0.05％，（3）0.1％和（4）0.15％普雷斯汀的基础饮食。在11天实验结束时，从仔猪收集回肠，肝脏，胸腺和脾样品。与对照组相比，0.15％的普雷斯特能显着提高抗羟基激进能力（回肠和脾脏），抗超氧化物阴离子容量（肝脏，胸腺和脾），过氧化氢酶（HELEUM，肝，胸腺和脾），总超氧化物歧化酶（回肠，胸腺和脾脏），谷胱甘肽过氧化物酶（回肠，肝脏和胸腺），谷胱甘肽S转移酶活性（回肠，肝脏，胸腺和脾），谷胱甘肽含量（肝脏和脾脏）和总抗氧化能力（回肠和胸腺）;减少丙二醛（回肠，肝，胸腺和脾），蛋白质羰基含量（回肠，肝，胸腺和脾）;增强Zonula occludens（ZO）-1（脾），ZO-2（肝，胸腺和脾），瘤素1（回肠，肝脏，胸腺和脾脏），克劳丁的mRNA表达2（回肠，胸腺和脾脏），Claudin 3（Hereum，肝脏，胸腺和脾），克劳丁14（回肠，肝脏和脾脏），克劳丁16（回肠和肝脏），超氧化物歧化酶1（Hereum，肝脏和胸腺），谷胱甘肽过氧化物酶1（回肠，肝脏，胸腺和脾脏），过氧化氢酶（Hereum，肝，胸腺和脾），谷胱甘肽还原酶（胸腺和脾脏），谷胱甘肽S-转移酶（回肠，肝脏，胸腺和脾脏）和核红虫2-相关因子2（肝脏和胸腺）;减少肌球蛋白轻链激酶（回肠，肝，胸腺和脾脏）和肝脏+相关蛋白1（肝脏和脾）的mRNA水平（P <0.05）。 0.05％Putrescine可以显着影响一些上述参数（P <0.05）。集体，Putrescine补充剂可提高器官的物理屏障功能和剂量依赖性和独立效应的物理屏障功能和抗氧化能力;这种改进对断奶仔猪的健康有益。

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  • 来源
    《RSC Advances》 |2019年第34期|共12页
  • 作者

    Liu Guangmang; Mo Weiwei; Xu Xiaomei; Wu Xianjian; Jia Gang; Zhao Hua; Chen Xiaoling; Wu Caimei; Wang Jing;

  • 作者单位

    Sichuan Agr Univ Inst Anim Nutr Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Anim Nutr Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Anim Nutr Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Anim Nutr Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Anim Nutr Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Anim Nutr Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Anim Nutr Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Anim Nutr Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Maize Res Inst Chengdu 611130 Sichuan Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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