• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Linker length in fluorophore-cholesterol conjugates directs phase selectivity and cellular localisation in GUVs and live cells
【24h】

Linker length in fluorophore-cholesterol conjugates directs phase selectivity and cellular localisation in GUVs and live cells

机译：荧光团中的接头长度缀合物缀合物引导uVVS和活细胞中的相选择性和细胞定位

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Lipid membrane fluorescent probes that are both domain-selective and compatible with demanding microscopy methods are crucial to elucidate the presence and function of rafts and domains in cells and biophysical models. Whereas targeting fluorescent probes to liquid-disordered (L-d) domains is relatively facile, it is far more difficult to direct probes with high selectivity to liquid-ordered (L-o) domains. Here, a simple, one-pot approach to probe-cholesterol conjugation is described using Steglich esterification to synthesise two identical BODIPY derivatives that differ only in the length of the aliphatic chain between the dye and cholesterol. In the first, BODIPY-Ar-Chol, the probe and cholesterol were directly ester linked and in the second BODIPY-Ahx-Chol, a hexyl linker separated probe from cholesterol. Uptake and distribution of each probe was compared in ternary, phase separated giant unilamellar vesicles (GUVs) using a commercial L-d marker as a reference. BODIPY-Ar-Chol targets almost exclusively the L-d domains with selectivity of >90% whereas by contrast introducing the C-6 linker between the probe and cholesterol drove the probe to L-o with excellent selectivity (>80%). The profound impact of the linker length extended also to uptake and distribution in live mammalian cells. BODIPY-Ahx-Chol associates strongly with the plasma membrane where it partitioned preferably into opposing micron dimensioned do-mains to a commercial L-d marker and its concentration at the membrane was reduced by cyclodextrin treatment of the cells. By contrast the BODIPY-Ahx-Chol permeated the membrane and localised strongly to lipid droplets within the cell. The data demonstrates the profound influence of linker length in cholesterol bioconjugates in directing the probe.
机译：脂质膜荧光探针与苛刻的显微镜方法相容是至关重要的，以阐明细胞和生物物理模型中的筏和结构域的存在和功能至关重要。然而，靶向荧光探针对液体无序（L-D）结构域相对容易，因此将具有高选择性与液体有序（L-O）结构域具有高选择性的探针更难以。这里，使用STEGLICH Esterification描述了一种简单的单壶探针 - 胆固醇缀合的方法，以合成两个相同的Bodipy衍生物，其仅在染料和胆固醇之间的脂族链的长度中不同。在第一，BODIPY-AR-CHOL中，探针和胆固醇是直接连接的酯，在第二BODIPY-AHX-CHOL中，来自胆固醇的己基接头分离探针。将每种探针的摄取和分布在三元相间，使用商业L-D标记作为参考的分离的巨型Unilamellar囊泡（GUV）进行比较。通过对比将L-D结构具有> 90％的L-D结构，而在探针和胆固醇之间引入L-O之间的C-6接头，具有优异的选择性（> 80％）的探针将探针与L-O之间的选择性> 90％的L-D结构靶向。接头长度的深刻影响延伸到活哺乳动物细胞中的摄取和分布。 BODIPY-AHX-CHOL与血浆膜强烈相关联，其中优选将其分配到与商业L-D标记相对的微米尺寸的DO-MARKER中，并且通过对细胞的环糊精处理降低了其在膜上的浓度。相反，BODIPY-AHX-CHOL渗透膜并强烈地局部地局限于细胞内的脂液滴。该数据证明了在引导探针中胆固醇生物缀合物中的接头长度的深刻影响。

著录项

  • 来源
    《RSC Advances》 |2019年第40期|共12页
  • 作者

    O Connor Darragh; Byrne Aisling; Keyes Tia E.;

  • 作者单位

    Dublin City Univ Natl Ctr Sensor Res Sch Chem Sci Dublin 9 Ireland;

    Dublin City Univ Natl Ctr Sensor Res Sch Chem Sci Dublin 9 Ireland;

    Dublin City Univ Natl Ctr Sensor Res Sch Chem Sci Dublin 9 Ireland;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号