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首页> 外文期刊>RSC Advances >Mn3O4 microspheres as an oxidase mimic for rapid detection of glutathione
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Mn3O4 microspheres as an oxidase mimic for rapid detection of glutathione

机译：Mn3O4微球作为氧化酶模拟，用于快速检测谷胱甘肽

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摘要

Exploiting a rapid and sensitive method for biomarker detection has important implications in the early diagnosis of diseases. Here, we synthesized Mn3O4 microspheres which worked as a nanozyme to exhibit outstanding oxidase-like activity for rapid colorimetric determination of glutathione (GSH). The Mn3O4 microspheres of about 800 nm in size could be prepared through a hydrothermal method, and we found that the as-prepared Mn3O4 microspheres could quickly oxidize 3,3 0,5,5 0 -tetramethylbenzidine (TMB) to its oxidized form (TMBox) in the absence of H2O2. After adding glutathione (GSH), TMBox was able to be changed into to its original form and resulted in the corresponding decrease in absorbance value at 652 nm. The Mn3O4-TMB system had good linearity with GSH concatenation in the range of 560 mM, and the limit of detection was 0.889 mM. Furthermore, this assay possessed high selectivity specificity, which made it possible to detect GSH in human serum samples. Thus, the obtained assay based on the oxidase mimic of Mn3O4 would enlarge and exploit the application fields of nanozymes in bio-analysis.
机译：利用迅速敏感的生物标志物检测方法对疾病的早期诊断具有重要意义。这里，我们合成了Mn3O4微球，其用作纳米沸点，以表现出优异的氧化酶样活性，用于快速上测量谷胱甘肽（GSH）。尺寸约为800nm的Mn3O4微球可以通过水热法制制备，我们发现制备的Mn 3 O 4微球可以将3,30,5,5,5,5. 0-二甲基苯（TMB）迅速氧化成其氧化形式（Tmbox ）在没有H2O2的情况下。在添加谷胱甘肽（GSH）之后，能够将Tmbox变成其原始形式，并导致652nm处的吸光度值的相应降低。 MN3O4-TMB系统具有良好的线性度，GSH连接在560mm的范围内，检测限为0.889 mm。此外，该测定具有高选择性特异性，这使得可以检测人血清样品中的GSH。因此，基于MN3O4的氧化酶模拟的测定将扩大并利用生物分析中纳佐的施用领域。

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  • 来源
    《RSC Advances》 |2019年第29期|共6页
  • 作者

    Xi Juqun; Zhu Chunhua; Wang Yanqiu; Zhang Qiannan; Fan Lei;

  • 作者单位

    Yangzhou Univ Inst Translat Med Dept Pharmacol Coll Med Yangzhou 225001 Jiangsu Peoples R China;

    Yangzhou Univ Inst Translat Med Dept Pharmacol Coll Med Yangzhou 225001 Jiangsu Peoples R China;

    Yangzhou Univ Inst Translat Med Dept Pharmacol Coll Med Yangzhou 225001 Jiangsu Peoples R China;

    Yangzhou Univ Inst Translat Med Dept Pharmacol Coll Med Yangzhou 225001 Jiangsu Peoples R China;

    Yangzhou Univ Sch Chem &

    Chem Engn Yangzhou 225002 Jiangsu Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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