Culturing and patch clamping of Jurkat T cells and neurons on Al2O3 coated nanowire arrays of altered morphology

Harberts Jann; Zierold Robert; Fendler Cornelius; Koitmaee Aune; Bayat Parisa; Fernandez-Cuesta Irene; Loers Gabriele; Diercks Bjoern-Philipp; Fliegert Ralf; Guse Andreas H.; Ronning Carsten; Otnes Gaute; Borgstrom Magnus; Blick Robert H.

首页> 外文期刊>RSC Advances >Culturing and patch clamping of Jurkat T cells and neurons on Al2O3 coated nanowire arrays of altered morphology

【24h】

Culturing and patch clamping of Jurkat T cells and neurons on Al2O3 coated nanowire arrays of altered morphology

机译：Jurkat T细胞和神经元在Al2O3涂覆纳米线阵列改变形态的培养和贴片

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Nanowire substrates play an increasingly important role for cell cultures as an approach for hybrid bio-semiconductor junctions. We investigate Jurkat T cells and neurons from mice cultured on Al2O3 coated ordered and randomly distributed nanowires. Cell viability was examined by life/membrane staining reporting comparable viability on planar and nanowire substrates. Imaging the hybrid interface reveals a wrapping of the cell membrane around the very nanowire tip. Patch clamp recordings show similar electrophysiological responses on each type of nanowires compared to planar control substrates. We demonstrate that the morphological characteristic of the nanowire substrate plays a subordinate role which opens up the arena for a large range of nanowire substrates in a functionalized application such as stimulation or sensing.

机译：纳米线基质对细胞培养物作为杂交生物半导体结的方法发挥着越来越重要的作用。我们研究了在Al2O3涂覆有序和随机分布的纳米线上培养的小鼠的Jurkat T细胞和神经元。通过寿命/膜染色报告平面和纳米线基质上的可比存活率检查细胞活力。成像混合界面揭示了细胞膜周围的纳米线尖端的包裹。与平面对照基板相比，贴片夹具录制在每种类型的纳米线上显示出类似的电生理响应。我们表明纳米线基板的形态特征在官能化施加诸如刺激或感测的官能化应用中，纳米线基材的形态学特性起到了从官能化的应用中的大范围纳米线基板的竞技场。

著录项

来源
《RSC Advances》 |2019年第20期|共8页
作者
Harberts Jann; Zierold Robert; Fendler Cornelius; Koitmaee Aune; Bayat Parisa; Fernandez-Cuesta Irene; Loers Gabriele; Diercks Bjoern-Philipp; Fliegert Ralf; Guse Andreas H.; Ronning Carsten; Otnes Gaute; Borgstrom Magnus; Blick Robert H.;
展开▼
作者单位

Univ Hamburg Ctr Hybrid Nanostruct Luruper Chaussee 149 D-22761 Hamburg Germany;

Univ Hamburg Ctr Hybrid Nanostruct Luruper Chaussee 149 D-22761 Hamburg Germany;

Univ Hamburg Ctr Hybrid Nanostruct Luruper Chaussee 149 D-22761 Hamburg Germany;

Univ Hamburg Ctr Hybrid Nanostruct Luruper Chaussee 149 D-22761 Hamburg Germany;

Univ Hamburg Ctr Hybrid Nanostruct Luruper Chaussee 149 D-22761 Hamburg Germany;

Univ Hamburg Ctr Hybrid Nanostruct Luruper Chaussee 149 D-22761 Hamburg Germany;

Univ Med Ctr Hamburg Eppendorf Ctr Mol Neurobiol Hamburg Falkenried 94 D-20251 Hamburg Germany;

Univ Med Ctr Hamburg Eppendorf Calcium Signaling Grp Dept Biochem &

Mol Cell Biol Martinistr 52 D-20251 Hamburg Germany;

Univ Med Ctr Hamburg Eppendorf Calcium Signaling Grp Dept Biochem &

Mol Cell Biol Martinistr 52 D-20251 Hamburg Germany;

Univ Med Ctr Hamburg Eppendorf Calcium Signaling Grp Dept Biochem &

Mol Cell Biol Martinistr 52 D-20251 Hamburg Germany;

Friedrich Schiller Univ Jena Inst Solid State Phys Helmholtzweg 3-5 D-07743 Jena Germany;

Lund Univ NanoLund Box 118 S-22100 Lund Sweden;

Lund Univ NanoLund Box 118 S-22100 Lund Sweden;

Univ Hamburg Ctr Hybrid Nanostruct Luruper Chaussee 149 D-22761 Hamburg Germany;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类化学;
关键词

相似文献

外文文献
中文文献
专利

1. Culturing and patch clamping of Jurkat T cells and neurons on Al2O3 coated nanowire arrays of altered morphology [J] . Harberts Jann, Zierold Robert, Fendler Cornelius, RSC Advances . 2019,第20期

机译：Jurkat T细胞和神经元在Al2O3涂覆纳米线阵列改变形态的培养和贴片
2. Culturing and patch clamping of Jurkat T cells and neurons on Al2O3 coated nanowire arrays of altered morphology [J] . Jann Harberts, Robert Zierold, Cornelius Fendler, RSC Advances . 2019,第20期

机译：在形态改变的Al2O3包覆的纳米线阵列上Jurkat T细胞和神经元的培养和膜片钳
3. Correction: Culturing and patch clamping of Jurkat T cells and neurons on Al2O3 coated nanowire arrays of altered morphology [J] . Jann Harberts, Robert Zierold, Cornelius Fendler, RSC Advances . 2019,第24期

机译：校正：Jurkat T细胞和神经元在形态改变的Al2O3涂层纳米线阵列上的培养和膜片钳
4. Secretion of different altered laminin isoforms results in three-dimensional morphological changes in cells cultured on Matrigel [C] . Imai Masayoshi, Mizutani Tomoko, Kawabata Kuniaki, International Symposium on Micro-NanoMechatronics and Human Science . 2014

机译：分泌不同的层粘连蛋白同工型会导致基质胶上培养的细胞发生三维形态变化
5. Patch-clamp analysis of peptidergic excitation in rat supraoptic neurons. [D] . Chakfe, Yassar. 1997

机译：大鼠超视神经元肽能兴奋的膜片钳分析。
6. A Temperature-Controlled Patch Clamp Platform Demonstrated on Jurkat T Lymphocytes and Human Induced Pluripotent Stem Cell-Derived Neurons [O] . Jann Harberts, Max Kusch, John O’Sullivan, 2020

机译：在Jurkat T淋巴细胞和人类诱导的多能干细胞衍生的神经元上展示了一个温度控制的膜片钳平台
7. Simultaneous multi-patch-clamp and extracellular-array recordings: Single neuron reflects network activity [O] . Vardi, Roni, Goldental, Amir, Sardi, Shira, 2017

机译：同时多片钳和细胞外阵列记录：单神经元反映网络活动

Culturing and patch clamping of Jurkat T cells and neurons on Al2O3 coated nanowire arrays of altered morphology

摘要

著录项

相似文献

相关主题

期刊订阅