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首页> 外文期刊>RSC Advances >Preparation of cellular samples using graphene cover and air-plasma treatment for time-of-flight secondary ion mass spectrometry imaging
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Preparation of cellular samples using graphene cover and air-plasma treatment for time-of-flight secondary ion mass spectrometry imaging

机译：使用石墨烯盖和空气等离子体处理的细胞样品的制备用于飞行时间二次离子质谱成像

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We report on sample preparation methods based on plasma treatment for an improvement of multiple molecular ion images of cellular membranes in the ToF-SIMS method. The air-plasma treatment of fixed cellular samples efficiently removed the organic residues of any solutions used during sample preparation and improved the quality of ToF-SIMS images due to the resulting clean surface. We also studied cell preparation methods that combine single-layer graphene covering with air-plasma treatment to achieve a synergistic effect that eliminates background spectra by organic impurities while minimizing morphological cell deformation in a vacuum environmental analysis. When the cellular sample on the glass substrate is completely covered with the single-layer graphene, the cells trapped between the graphene and the substrate can effectively reduce morphological deformation by slow-dehydration. After slow-dehydration of cells is completed inside the graphene-cover, the covered graphene layer can be peeled off by air-plasma treatment, and the unwanted organic residues on the surface of cells and substrate can also be removed by plasma cleaning, thereby much improving ion imaging of cells with the ToF-SIMS method. It is confirmed that the cell samples in which the graphene-cover was removed by air-plasma treatment maintained their morphology well in comparison with the rapid air-dried cells in atomic force microscopy (AFM) and ToF-SIMS images.
机译：我们报告基于等离子体处理的样品制备方法，以改善TOF-SIMS方法中的细胞膜多重分子离子图像。固定细胞样品的空气等离子体处理有效地除去样品制备期间使用的任何溶液的有机残留物，并通过所得到的清洁表面改善TOF-SIMS图像的质量。我们还研究了细胞制备方法，该方法将单层石墨烯覆盖物与空气等离子体处理相结合，实现协同作用，以通过有机杂质消除背景光谱，同时在真空环境分析中最小化形态细胞变形。当玻璃基板上的细胞样品完全覆盖单层石墨烯时，捕获在石墨烯和基材之间的细胞可以通过缓慢脱水有效地降低形态变形。在石墨烯内部完成细胞缓慢脱水后，覆盖的石墨烯层可以通过空气等离子体处理剥离，并且通过等离子体清洁也可以去除细胞和基材表面上的不需要的有机残留物，从而大大用TOF-SIMS方法改善细胞离子成像。确实证实，通过空气等离子体处理除去石墨烯盖的细胞样品，与原子力显微镜（AFM）和TOF-SIMS图像中的快速风干细胞相比，它们的形态保持良好。

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    《RSC Advances》 |2019年第49期|共7页
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  • 正文语种 eng
  • 中图分类 化学;
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