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首页> 外文期刊>RSC Advances >The size of micro-crystalline tyrosine (MCT (R)) influences its recognition and uptake by THP-1 macrophages in vitro
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The size of micro-crystalline tyrosine (MCT (R)) influences its recognition and uptake by THP-1 macrophages in vitro

机译：微晶酪氨酸的大小（MCT（R））对THP-1巨噬细胞体外影响其识别和吸收

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The physicochemical hallmarks of particulate immunopotentiators play a pivotal role with regards to their adjuvanticity in vivo. These properties have not been fully characterised in the case of MCT (R), an amino acid-based adjuvant used as an alternative to aluminium salts in subcutaneous allergy immunotherapy (SCIT). This study presents a full characterisation of MCT (R) and in a preliminary capacity reveals how parameters, specifically particle size, might influence the recognition of MCT (R) by antigen presenting cells (APCs) in vitro. Light microscopic analysis demonstrated that MCT (R) was composed of highly crystalline needles, the majority of which exceeded 10 mu m in length under physiological conditions (median size - 20.8 mu m). While the substantial length of crystals presented a significant barrier to cellular recognition and uptake, isolated incidences of perpendicular recognition were observed owing to the smaller comparative width of crystallites (median size - 2.8 mu m). This appeared to allow a small proportion of material to be ingested both fully and partially by THP-1 macrophages, although further studies are required to unequivocally confirm this observation. Preferential recognition of needle tips also favoured the direct presentation of antigen to immune cells as proteinaceous adsorption appeared to be isolated to these regions. Furthermore, the data herein provide valuable insights into the mechanisms surrounding how this adjuvant potentiates an immunological response following administration.
机译：颗粒状免疫脑的物理化学标志在体内佐利的佐治性方面发挥了关键作用。在MCT（R）的情况下，这些性质尚未完全表征，氨基酸基佐剂用作皮下过敏免疫疗法（SCIT）中的铝盐的替代物。该研究呈现了MCT（R）的完全表征，并且初步容量揭示了参数，具体粒度的方式如何影响体外抗原呈递细胞（APC）的MCT（R）的识别。光学显微镜分析证明MCT由高度结晶针组成，其中大多数在生理条件下超过10μm的长度（中位数 - 20.8μm）。虽然大量晶体呈现对细胞识别和摄取的显着屏障，但由于微晶的比较宽度较小（中位数 - 2.8μm），观察到垂直识别的分离出分离的术语。这似乎允许通过THP-1巨噬细胞完全和部分地摄取少量的材料，尽管需要进一步的研究以明确证实这种观察。针尖优先识别也赞成抗原直接呈现免疫细胞，因为蛋白质吸附似乎被分离为这些区域。此外，本文的数据提供了对围绕该佐剂如何增强给药后免疫应答的机制的有价值的见解。

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    《RSC Advances》 |2019年第42期|共14页
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  • 正文语种 eng
  • 中图分类 化学;
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