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首页> 外文期刊>RSC Advances >Ultrasensitive detection of Staphylococcal enterotoxin B in milk based on target-triggered assembly of the flower like nucleic acid nanostructure
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Ultrasensitive detection of Staphylococcal enterotoxin B in milk based on target-triggered assembly of the flower like nucleic acid nanostructure

机译：基于核酸纳米核酸甲醛靶触发组装的牛奶葡萄球菌肠毒素B的超声检测

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A rapid and ultrasensitive method is described for the detection of Staphylococcal enterotoxin B (SEB). It is based on the formation of the flower like nucleic acid nanostructure by integrating (a) target-induced triggering of DNA release with (b) signal amplification by a hybridization chain reaction (HCR). Firstly, partially complementary pairing of aptamer and trigger DNA forms a duplex structure. The capture DNA (cpDNA) is then placed on the surface of gold electrode through gold-thiol chemistry. In the presence of SEB, the aptamer-target conjugate is compelled to form. This causes the release of trigger DNA owing to a strong competition between aptamer and SEB. Then, the trigger DNA is subsequently hybridized with the partial complementary sequences of the cpDNA to trigger HCR with three auxiliary DNA sequences (referred to as MB1, MB2, MB3). Finally, the flower like nucleic acid nanostructures are formed and allow numerous hexaammineruthenium(iii) chloride ([Ru(NH3)(6)](3+), RuHex) to be absorbed on the DNA by electrostatic interaction, and thus amplify electrochemical signal. Under optimal conditions, the chronocoulometry charge difference increases linearly with the logarithm of the SEB concentrations in the range from 5 pg mL(-1) to 100 ng mL(-1) with a detection limit as low as 3 pg mL(-1) (S/N = 3).
机译：描述了一种用于检测葡萄球菌肠毒素B（SEB）的快速和超细方法。通过将（a）通过杂交链反应（HCR）与（b）信号扩增的靶诱导的DNA释放触发（a）靶诱导的DNA释放触发，基于核酸纳米结构的形成。首先，部分互补的适体配对和触发DNA形成双链体结构。然后通过金 - 硫醇化学将捕获DNA（CPDNA）置于金电极的表面上。在SEB的存在下，将适体 - 靶缀合物被迫使形成。由于适体和SEB之间的强烈竞争，这导致触发DNA的释放。然后，随后将触发DNA与CPDNA的部分互补序列杂交，以用三种辅助DNA序列触发HCR（称为MB1，MB2，MB3）。最后，形成核酸纳米结构的花并允许通过静电相互作用在DNA上被吸收在DNA上的许多六丙烯酸丁烯（III）（[Ru（NH 3）（6），RuHex），从而扩增电化学信号。在最佳条件下，刻度管测定电荷差与SEB浓度在5pg ml（-1）至100ng ml（-1）的范围内线性增加，检测极限为低至3 pg ml（-1） （s / n = 3）。

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    《RSC Advances》 |2019年第72期|共7页
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  • 正文语种 eng
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