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首页> 外文期刊>RSC Advances >Effect of resveratrol on drug resistance in colon cancer chemotherapy
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Effect of resveratrol on drug resistance in colon cancer chemotherapy

机译：白藜芦醇对结肠癌化疗耐药性的影响

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摘要

To investigate the effects of resveratrol on the drug resistance of 5-FU in the colon cancer chemotherapy, an MTT assay was used to detect the effects of 5-FU and resveratrol combined with 5-FU on the proliferation of the LoVo and SW480 cell lines. Flow cytometry was used to detect the effect of 5-FU combined with resveratrol on the survival rate of the LoVo and SW480 cells. A western blot was used to detect the expression levels of the proteins associated with colon cancer. After flow sorting, the percentage of the SW480 and the LoVo cell line CD133(+) was 97.5% and 95.8%, respectively. The cells cultured in vitro showed more rapid cell proliferation and differentiation. The MTT assay showed that as compared with the survival rate of the blank group LoVo and CD133+ LoVo cells, the survival rate of the cells containing the 5-FU group was lower (P 0.05). When 5-FU was used in combination with different concentrations of resveratrol, the abovementioned phenomenon was more prominent. The sorted colon cancer cells have dry stem cells, and the sorted CD133(+) cells are more resistant to drugs; the combination of resveratrol and 5-FU has the best effect on the colon cancer cells. Preliminary studies on the mechanism of action of the drug show that a combination of 5-FU and resveratrol regulates apoptosis in CD133(+) colon cancer stem cells by regulating the BAX gene; however, more complex mechanisms may also be involved.
机译：为了探讨白藜芦醇对结肠癌化疗中5-FU耐药性的影响，使用MTT测定来检测5-FU和白藜芦醇与5-FU联合对LOVO和SW480细胞系的增殖的影响。流式细胞术用于检测5-FU与白藜芦醇结合在LOVO和SW480细胞的存活率中的作用。用于检测与结肠癌相关的蛋白质的表达水平。流动分选后，SW480和Lovo细胞系CD133（+）的百分比分别为97.5％和95.8％。体外培养的细胞显示出更快速的细胞增殖和分化。 MTT测定表明，与空白组Lovo和CD133 + Lovo细胞的存活率相比，含有5-FU组的细胞的存活率较低（P <0.05）。当5-FU与不同浓度的白藜芦醇组合使用时，上述现象更加突出。分选的结肠癌细胞具有干燥干细胞，分选的CD133（+）细胞更耐药;白藜芦醇和5-FU的组合对结肠癌细胞具有最佳影响。对药物作用机制的初步研究表明，通过调节BAX基因，5-FU和白藜芦醇的组合调节CD133（+）结肠癌干细胞的细胞凋亡;然而，也可以涉及更复杂的机制。

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  • 来源
    《RSC Advances》 |2019年第5期|共9页
  • 作者

    Huang Lu; Zhang Sheng; Zhou Jun; Li Xiangzhou;

  • 作者单位

    Cent South Univ Forestry &

    Technol Sch Mat Sci &

    Engn 498 South Shaoshan Ave Changsha 410004 Hunan Peoples R China;

    Cent South Univ Forestry &

    Technol Sch Mat Sci &

    Engn 498 South Shaoshan Ave Changsha 410004 Hunan Peoples R China;

    Cent South Univ Forestry &

    Technol Sch Mat Sci &

    Engn 498 South Shaoshan Ave Changsha 410004 Hunan Peoples R China;

    Cent South Univ Forestry &

    Technol Sch Mat Sci &

    Engn 498 South Shaoshan Ave Changsha 410004 Hunan Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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