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首页> 外文期刊>RSC Advances >Polydopamine-modified poly(l-lactic acid) nanofiber scaffolds immobilized with an osteogenic growth peptide for bone tissue regeneration
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Polydopamine-modified poly(l-lactic acid) nanofiber scaffolds immobilized with an osteogenic growth peptide for bone tissue regeneration

机译：用骨组织再生的骨组织再生成骨化生长肽固定聚二匹马胺改性聚（L-乳酸）纳米纤维支架

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摘要

It is highly desirable for bone tissue engineering scaffolds to have significant osteogenic properties and capability to improve cell growth and thus enhance bone regeneration. In this study, a poly(l-lactic acid) (PLLA) nanofiber scaffold-immobilized osteogenic growth peptide (OGP) was prepared via polydopamine (PDA) coating. X-ray photoelectron spectroscopy (XPS), contact angle measurement, and scanning electron microscopy (SEM) were used to determine the OGP immobilization, hydrophilicity and surface roughness of the samples. The SEM and fluorescence images demonstrate that the PLLA nanofiber scaffolds immobilized with the OGP have excellent cytocompatibility in terms of cell adhesion and proliferation. The ALP activity and the Runx2 and OPN expression results indicated that the PLLA nanofiber scaffolds immobilized with OGP significantly enhanced the osteogenic differentiation and calcium mineralization of hMSCs in vitro. A rat model of critical skull bone defect was selected to evaluate the bone formation capacity of the scaffolds. Micro CT analysis and histological results demonstrated that the PLLA scaffolds immobilized with OGP significantly promoted bone regeneration in critical-sized bone defects. This study verifies that the PLLA scaffold-immobilized OGP has significant potential in bone tissue engineering.
机译：对于骨组织工程支架非常理想，具有显着的成骨性能和能力，以改善细胞生长，从而提高骨再生。在该研究中，通过多碳胺（PDA）涂层制备聚（L-乳酸）（PLLA）纳米纤维支架固定的骨质发生肽（OGP）。 X射线光电子能谱（XPS），接触角测量和扫描电子显微镜（SEM）用于确定样品的OGP固定，亲水性和表面粗糙度。 SEM和荧光图像表明，在细胞粘附和增殖方面，固定在OGP的PLLA纳米纤维支架具有优异的细胞偶联性。 ALP活性和RUNX2和OPN表达结果表明，用OGP固定的PLLA纳米纤维支架显着提高了体外HMSCs的骨质发生分化和钙矿化。选择临界头骨缺损的大鼠模型，评价支架的骨形成能力。微型CT分析和组织学结果表明，用OGP固定的PLLA支架显着促进了临界大小骨缺损中的骨再生。本研究验证了PLLA支架固定的OGP在骨组织工程中具有显着潜力。

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  • 来源
    《RSC Advances》 |2019年第21期|共15页
  • 作者

    Liu Yong; Xu Changlu; Gu Yong; Shen Xiaofeng; Zhang Yanxia; Li Bin; Chen Liang;

  • 作者单位

    Soochow Univ Affiliated Hosp 1 Dept Orthopaed Surg Suzhou 215006 Jiangsu Peoples R China;

    Soochow Univ Affiliated Hosp 1 Dept Orthopaed Surg Suzhou 215006 Jiangsu Peoples R China;

    Soochow Univ Affiliated Hosp 1 Dept Orthopaed Surg Suzhou 215006 Jiangsu Peoples R China;

    Nanjing Univ Chinese Med Suzhou TCM Hosp Nanjing Jiangsu Peoples R China;

    Soochow Univ Affiliated Hosp 1 Inst Cardiovasc Sci Suzhou 215007 Jiangsu Peoples R China;

    Soochow Univ Orthoped Inst Suzhou 215007 Jiangsu Peoples R China;

    Soochow Univ Affiliated Hosp 1 Dept Orthopaed Surg Suzhou 215006 Jiangsu Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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