• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Detection of streptavidin-biotin intermediate metastable states at the single-molecule level using high temporal-resolution atomic force microscopy
【24h】

Detection of streptavidin-biotin intermediate metastable states at the single-molecule level using high temporal-resolution atomic force microscopy

机译：使用高时分辨率原子力显微镜检测单分子水平的链霉抗生物素蛋白 - 生物素中间亚稳态

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Although the streptavidin-biotin intermolecular bond has been extensively used in many applications due to its high binding affinity, its exact nature and interaction mechanism have not been well understood. Several reports from previous studies gave a wide range of results in terms of the system's energy potential landscape because of bypassing some short-lived states in the detection process. We employed a quasi-static process of slowly loading force onto the bond (loading rate = 20 pN s(-1)) to minimize the force-induced disruption and to provide a chance to explore the system in near-equilibrium. Therein, by utilizing a fast sampling rate for the detection of force by atomic force microscopy (20 mu s per data point), several transient states of the system were clearly resolved in our force spectroscopy measurements. These key strategies allow the determination of the states' relative positions and free energy levels along the pulling reaction coordinate for the illustration of an energy landscape of the system.
机译：尽管在许多应用中，链霉抗生物素蛋白 - 生物素分子间键在许多应用中被广泛使用，但其确切的性质和相互作用机理尚未得到很好的理解。以前研究的几个报告在系统的能量潜在景观方面提供了广泛的结果，因为绕过了在检测过程中的一些短期状态。我们采用了一种缓慢加载力的准静态过程在键上（装载速率= 20 pn s（-1）），以最小化力引起的中断，并提供探索近平衡系统的机会。其中，通过利用原子力显微镜检测力的快速采样率（每个数据点20μs），在我们的力光谱测量中清楚地解决了系统的几个瞬态状态。这些关键策略允许沿着拉伸反应坐标确定状态的相对位置和自由能级，用于说明系统的能量景观。

著录项

  • 来源
    《RSC Advances》 |2019年第39期|共8页
  • 作者

    Mondarte Evan Angelo; Maekawa Tatsuhiro; Nyu Takashi; Tahara Hiroyuki; Lkhamsuren Ganchimeg; Hayashi Tomohiro;

  • 作者单位

    Tokyo Inst Technol Sch Mat &

    Chem Technol Dept Mat Sci &

    Engn Midori Ku 4259 Nagatsuta Cho Yokohama Kanagawa 2268503 Japan;

    Tokyo Inst Technol Sch Mat &

    Chem Technol Dept Mat Sci &

    Engn Midori Ku 4259 Nagatsuta Cho Yokohama Kanagawa 2268503 Japan;

    Tokyo Inst Technol Sch Mat &

    Chem Technol Dept Mat Sci &

    Engn Midori Ku 4259 Nagatsuta Cho Yokohama Kanagawa 2268503 Japan;

    Tokyo Inst Technol Sch Mat &

    Chem Technol Dept Mat Sci &

    Engn Midori Ku 4259 Nagatsuta Cho Yokohama Kanagawa 2268503 Japan;

    Tokyo Inst Technol Sch Mat &

    Chem Technol Dept Mat Sci &

    Engn Midori Ku 4259 Nagatsuta Cho Yokohama Kanagawa 2268503 Japan;

    Tokyo Inst Technol Sch Mat &

    Chem Technol Dept Mat Sci &

    Engn Midori Ku 4259 Nagatsuta Cho Yokohama Kanagawa 2268503 Japan;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号