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首页> 外文期刊>RSC Advances >In vitro evaluation of anti-hepatoma activity of brevilin A: involvement of Stat3/Snail and Wnt/beta-catenin pathways
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In vitro evaluation of anti-hepatoma activity of brevilin A: involvement of Stat3/Snail and Wnt/beta-catenin pathways

机译：Brevilin抗肝癌活性的体外评价A：Stat3 /蜗牛和WNT /β-连环蛋白途径的参与

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摘要

Brevilin A, a natural sesquiterpene lactone extracted from Centipeda minima, has been found to exhibit anti-tumor effect. However, the roles of brevilin A on hepatocellular carcinoma (HCC) have not yet been reported. The aim of the present study was to investigate the role of brevilin A in HCC and the underlying in vitro mechanisms. The HCC cell lines, HepG2 and SMMC-7221, were treated with different concentrations of brevilin A (0 mu M, 2.5 mu M, 5 mu M, 10 mu M, 15 mu M, and 20 mu M) for 48 h. MTT assay was performed to detect the cell viability. Flow cytometry was performed to detect cell apoptosis. Cell invasion was detected using the transwell assay. The expressions of matrix metalloproteinase (MMP)-2, MMP-9, phospho-signal transducer and activator of transcription 3 (p-Stat3), Stat3, Snail, beta-catenin, and c-Myc were detected using the western blot analysis. The results showed that brevilin A reduced cell viability and invasion in HepG2 and SMMC-7221 cells. The apoptotic rates of HepG2 and SMMC-7221 cells treated with brevilin A were found to be markedly increased. The expression levels of MMP-2 and MMP-9 were decreased after the treatment with brevilin A. In addition, brevilin A suppressed the Stat3/Snail and Wnt/beta-catenin signaling pathways in HCC cells. Collectively, brevilin A displayed an anti-tumor effect against HCC in vitro, which might be attributed to the inactivation of Stat3/Snail and Wnt/beta-catenin signaling pathways.
机译：已发现从Centipeda Minima提取的天然倍二萜内酯的Brevilin A，已发现抗肿瘤作用。然而，Brevilin A对肝细胞癌（HCC）的作用尚未报告。本研究的目的是探讨Brevilin A在HCC中的作用和潜在的体外机制。 HEC蜂窝线HepG2和SMMC-7221被不同浓度的Brevilina（0μm，2.5μm，5μm，10μm，15μm，20μm）处理48小时。进行MTT测定以检测细胞活力。进行流式细胞术以检测细胞凋亡。使用Transwell测定检测细胞侵袭。使用Western印迹分析检测了基质金属蛋白酶（MMP）-2，MMP-9，磷光信号传感器和转录3（P-STAT3），STAT3，蜗牛，β-连环蛋白和C-MYC的表达。结果表明，Beverilin在HepG2和SMMC-7221细胞中降低的细胞活力和侵袭。发现用Brevilin A处理的HepG2和SMMC-7221细胞的凋亡率明显增加。在用Brevilin A处理后，MMP-2和MMP-9的表达水平降低。另外，Brevilin A抑制了HCC细胞中的STAT3 /蜗牛和WNT /β-连环蛋白信号传导途径。共同，Brevilin A在体外展示了对HCC的抗肿瘤作用，这可能归因于STAT3 /蜗牛和WNT /β-连环蛋白信号传导途径的灭活。

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  • 来源
    《RSC Advances》 |2019年第8期|共7页
  • 作者

    Qin Yaguang; Lu Hong;

  • 作者单位

    Henan Univ Huaihe Hosp Dept Oncol 8 Baobei Rd Kaifeng 475000 Henan Peoples R China;

    Henan Univ Huaihe Hosp Dept Oncol 8 Baobei Rd Kaifeng 475000 Henan Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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