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Sensitive Raman detection of human recombinant interleukin-6 mediated by DCDR/GERS hybrid platforms

机译：DCDR / GERS混合平台介导人重组白细胞介素-6的敏感拉曼检测

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Recombinant human interleukin-6 (IL-6) is a key cytokine that plays an important role in the immune system and inflammatory response, explaining why any modification of its concentration in biological fluids is considered a signal of a pathological condition. Therefore, it is important to develop alternative, highly sensitive and reliable analytical methodologies to detect and identify this analyte in biological fluids. Herein, we present a proof of concept for the development of a new analytical hybrid platform for IL-6 detection that is based on the combination of drop-coating deposition Raman (DCDR) spectroscopy and graphene-enhanced Raman spectroscopy (GERS) effects. The sensitivity limits for IL-6 detection were found to be a function of the type of substrate used. When a 1 L droplet of IL-6 solution is deposited and dried on an Si substrate, a DCDR effect occurs, and a detection limit below 1 ng mL(-1) is obtained; however, when the same is performed using a hybrid substrate of reduced graphene oxide and silicon (rGO/Si), the joint action of DCDR and GERS effects results in a detection limit well below 1 pg mL(-1). It is important to note that this result implies the absolute mass detection of 1 fg of IL-6. In summary, the Raman spectroscopy DCDR/GERS analytical platform proposed here allows the reliable identification of, as well as the very sensitive detection of, IL-6 and promises to improve the performance of clinical evaluations of this biomarker that are currently in use. In this study, the Raman spectra of IL-6 in powder and solution, together with the corresponding band assignment, are presented for the first time in the literature.
机译：重组人白细胞介素-6（IL-6）是一种关键的细胞因子，其在免疫系统和炎症反应中起重要作用，解释了为什么任何在生物流体中浓度的修饰被认为是病理条件的信号。因此，重要的是要开发替代，高度敏感和可靠的分析方法，以检测和鉴定生物流体中的该分析物。在此，我们展示了用于开发用于IL-6检测的新分析混合平台的概念证据，其基于滴涂层沉积拉曼（DCDR）光谱和石墨烯增强的拉曼光谱（GERS）效应的组合。发现IL-6检测的灵敏度限制是所使用的基板类型的函数。当在Si衬底上沉积并干燥IL-6溶液的1L液滴时，发生DCDR效应，获得低于1ng（-1）的检测极限;然而，当使用异物氧化物和硅（RGO / Si）的混合衬底进行相同的时间，DCDR和GER​​效应的关节作用导致良好的检测限低于1pg ml（-1）。值得注意的是，该结果意味着1 FG的IL-6的绝对质量检测。总之，这里提出的拉曼光谱DCDR / GER分析平台允许可靠的鉴定以及IL-6的非常敏感的检测，并承诺改善目前正在使用的这种生物标志物的临床评价的性能。在该研究中，在文献中首次呈现粉末和溶液中IL-6的拉曼光谱与相应的频带分配一起呈现。

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  • 来源
    《RSC Advances》 |2019年第22期|共7页
  • 作者

    de la O-Cuevas Emmanuel; Badillo-Ramirez Isidro; Islas Selene R.; Araujo-Andrade C.; Saniger Jose M.;

  • 作者单位

    Univ Autonoma Zacatecas Unidad Acad Fis Zacatecas 98068 Mexico;

    Univ Nacl Autonoma Mexico Inst Ciencias Aplicadas &

    Tecnol Circuito Externo S-N Cd Univ Mexico City 04510 DF Mexico;

    Univ Nacl Autonoma Mexico Inst Ciencias Aplicadas &

    Tecnol Circuito Externo S-N Cd Univ Mexico City 04510 DF Mexico;

    Univ Autonoma Zacatecas Unidad Acad Fis Zacatecas 98068 Mexico;

    Univ Nacl Autonoma Mexico Inst Ciencias Aplicadas &

    Tecnol Circuito Externo S-N Cd Univ Mexico City 04510 DF Mexico;

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  • 正文语种 eng
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