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Conformational-transited protein corona regulated cell-membrane penetration and induced cytotoxicity of ultrasmall Au nanoparticles

机译：构象过渡的蛋白质电晕调节的细胞膜膜渗透和诱导超γA纳米粒子的细胞毒性

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摘要

Nanoparticles (NP) in biological fluids almost invariably become coated with proteins to form protein coronas. It is the NP-protein corona rather than the bare nanoparticle that determines the nanoparticle's bio-behavior. Here, ultrasmall gold nanoparticles (AuNPs) coated by a human serum albumin (HSA) corona were studied by Fourier transform infrared spectroscopy, denature experiments, fluorescence quenching. Moreover, the intracellular fate of AuNPs and the AuNP-HSA corona has also been investigated. The results show that HSA corona undergo a conformational transition (partial beta-sheet changed to alpha-helicity) when they adsorb on AuNPs, which lead to an enhanced thermal stability. Importantly, we observed that the conformation-transited protein corona-AuNP complex could induce cell apoptosis. Meanwhile, for the first time, the conformation-transited HSA on the AuNPs surface are shown to disrupt living cell membranes. The results obtained here not only provide the detailed conformational behavior of HSA molecules on nanoparticles, but also reveal the structure-function relationship of protein corona, which is of utmost importance in the safe application of nanoscale objects in living organisms.
机译：生物液中的纳米颗粒（NP）几乎总是与蛋白质涂覆以形成蛋白质coronas。它是NP-蛋白质电晕而不是确定纳米颗粒的生物行为的裸纳米粒子。这里，通过傅里叶变换红外光谱，变性实验，荧光猝灭，研究了由人血清白蛋白（HSA）电晕涂覆的超高氨基纳米颗粒（AUNP）。此外，还研究了AUNP和AUNP-HSA电晕的细胞内命运。结果表明，当它们吸附在AuNP上时，HSA电晕经历了构象过渡（改变为α-螺旋的部分β-薄片），这导致了增强的热稳定性。重要的是，我们观察到锥形过渡的蛋白质电晕 - aUnp复合物可以诱导细胞凋亡。同时，首次，AUNPS表面上的构象过渡的HSA被显示为破坏活细胞膜。这里获得的结果不仅在纳米颗粒上提供了HSA分子的详细构象行为，而且揭示了蛋白质电晕的结构功能关系，这对于生物体中的纳米级目物安全应用至关重要。

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  • 来源
    《RSC Advances》 |2019年第8期|共10页
  • 作者

    Yang Huayan; Wang Meng; Zhang Yanmin; Li Feng; Yu Shaoning; Zhu Lin; Guo Yuming; Yang Lin; Yang Shouning;

  • 作者单位

    Henan Normal Univ Key Lab Green Chem Media &

    React Sch Chem &

    Chem Engn Minist Educ Collaborat Innovat Ctr Henan Prov Green Mfg Fine Xinxiang Peoples R China;

    Henan Normal Univ Key Lab Green Chem Media &

    React Sch Chem &

    Chem Engn Minist Educ Collaborat Innovat Ctr Henan Prov Green Mfg Fine Xinxiang Peoples R China;

    Henan Normal Univ Key Lab Green Chem Media &

    React Sch Chem &

    Chem Engn Minist Educ Collaborat Innovat Ctr Henan Prov Green Mfg Fine Xinxiang Peoples R China;

    Univ Shanghai Sci &

    Technol Shanghai Peoples R China;

    Fudan Univ Shanghai Peoples R China;

    Henan Normal Univ Key Lab Green Chem Media &

    React Sch Chem &

    Chem Engn Minist Educ Collaborat Innovat Ctr Henan Prov Green Mfg Fine Xinxiang Peoples R China;

    Henan Normal Univ Key Lab Green Chem Media &

    React Sch Chem &

    Chem Engn Minist Educ Collaborat Innovat Ctr Henan Prov Green Mfg Fine Xinxiang Peoples R China;

    Henan Normal Univ Key Lab Green Chem Media &

    React Sch Chem &

    Chem Engn Minist Educ Collaborat Innovat Ctr Henan Prov Green Mfg Fine Xinxiang Peoples R China;

    Henan Normal Univ Key Lab Green Chem Media &

    React Sch Chem &

    Chem Engn Minist Educ Collaborat Innovat Ctr Henan Prov Green Mfg Fine Xinxiang Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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