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Rapid increase in transparency of biological organs by matching refractive index of medium to cell membrane using phosphoric acid

机译：使用磷酸将培养基折射率与细胞膜匹配通过磷酸的透明度快速增加

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摘要

Tissue clearing is a fundamental challenge in biology and medicine to achieve high-resolution optical imaging of tissues deep inside intact organs. The clearing methods reported up to now require long incubation times or physical/electrical pressure to achieve tissue clearing, which is done by matching the refractive indices of the whole sample and medium to that of the lipid layer. Here we show that phosphoric acid increases the refractive index of the medium and can increase the transparency of formalin-fixed tissue samples rapidly. While phosphoric acid (8.5-14.2 M) suppresses bright signals on the boundary of cells in their phase-contrast images, it does not damage the morphology of the phospholipid cell membrane. Immersion of fixed tissues of mice in phosphoric acid solutions (8.5-14.2 M) increased their transparency within 60 min in the case of 3 mm-thick fixed tissue specimens. Although further investigations are needed to apply this protocol to three-dimensional fluorescence imaging or immunohistochemistry, the protocol presented herein may contribute to developing better and faster soaking methods for tissue clearing than previously reported protocols.
机译：组织清除是生物学和药物的基本挑战，以实现内部内部内部的组织的高分辨率光学成像。报告的清算方法现在需要长孵育时间或物理/电压来实现组织清除，这通过将整个样品和培养基的折射率与脂质层的折射率匹配来实现。在这里，我们表明磷酸增加了培养基的折射率，可以迅速增加福尔马林固定组织样品的透明度。虽然磷酸（8.5-14.2M）抑制在其相位对比图像中细胞边界上的亮信号，但不会损害磷脂细胞膜的形态。在3mm厚的固定组织标本的情况下浸入磷酸溶液中的固定组织（8.5-14.2m）在60分钟内增加它们的透明度。尽管需要进一步调查来将该方案应用于三维荧光成像或免疫组织化学，但本文提出的方案可能有助于显影比以前报道的方案的组织清除更好，更快的浸泡方法。

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  • 来源
    《RSC Advances》 |2019年第27期|共8页
  • 作者

    Umezawa Masakazu; Haruguchi Shinsuke; Fukushima Rihito; Sekiyama Shota; Kamimura Masao; Soga Kohei;

  • 作者单位

    Tokyo Univ Sci Dept Mat Sci &

    Technol Fac Ind Sci &

    Technol Katsushika Ku 6-3-1 Niijuku Tokyo 1258585 Japan;

    Tokyo Univ Sci Dept Mat Sci &

    Technol Fac Ind Sci &

    Technol Katsushika Ku 6-3-1 Niijuku Tokyo 1258585 Japan;

    Tokyo Univ Sci Dept Mat Sci &

    Technol Fac Ind Sci &

    Technol Katsushika Ku 6-3-1 Niijuku Tokyo 1258585 Japan;

    Tokyo Univ Sci Dept Mat Sci &

    Technol Fac Ind Sci &

    Technol Katsushika Ku 6-3-1 Niijuku Tokyo 1258585 Japan;

    Tokyo Univ Sci Dept Mat Sci &

    Technol Fac Ind Sci &

    Technol Katsushika Ku 6-3-1 Niijuku Tokyo 1258585 Japan;

    Tokyo Univ Sci Dept Mat Sci &

    Technol Fac Ind Sci &

    Technol Katsushika Ku 6-3-1 Niijuku Tokyo 1258585 Japan;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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