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首页> 外文期刊>RSC Advances >Structural characterization and immunoregulatory activity of a new polysaccharide from Citrus medicaL. var. sarcodactylis
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Structural characterization and immunoregulatory activity of a new polysaccharide from Citrus medicaL. var. sarcodactylis

机译：柑橘医学新多糖的结构表征及免疫调节活性。 var。 sarcodactylis.

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摘要

A new homogeneous heteropolysaccharide (CMSPA90-1) was purified from bergamot by DEAE sepharose fast flow and Sephadex G-75 columns, and was shown to have a molecular weight of 17.6 kDa. Its chemical structure was elucidated by acid hydrolysis and methylation analysis, along with high-performance anion-exchange chromatography, Fourier transform infrared spectroscopy coupled with gas chromatography-mass spectrometry, NMR spectroscopies, the Congo red test, and circular dichroism. CMSPA90-1 consisted of a pyranoside and funanside with branches containing - and -configurations simultaneously. Arabinose and glucose might form an arabinoglucan backbone. The ultrastructure of CMSPA90-1 was further characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The results of thermogravimetric analysis (TGA) revealed that CMSPA90-1 had good thermal stability. The results of DPPH and ABTS(+) radical scavenging assays indicated that CMSPA90-1 exhibited free-radical-scavenging properties. Otherwise, CMSPA90-1 could promote the proliferation of mouse splenocytes and the neutral red phagocytosis of RAW264.7 cells, which indicated that CMSPA90-1 could be researched and developed as one of the potential functional foods or natural medicines.
机译：通过DEAE Sepharose快速流动和Sephadex G-75柱从佛手柑纯化新的均相杂多糖（CMSPA90-1），并显示出分子量为17.6kDa。其化学结构通过酸水解和甲基化分析阐明，以及高性能阴离子交换色谱，傅里叶变换红外光谱，与气相色谱 - 质谱，NMR光谱，刚果红色试验和圆形二色性耦合。 CMSPA90-1由吡喃烃和氟烷寡在同时含有含有的分支和 - 分支的组成。阿拉伯糖和葡萄糖可能形成阿拉伯葡萄糖骨架。通过扫描电子显微镜（SEM）和原子力显微镜（AFM），进一步表征CMSPA90-1的超微结构。热重分析（TGA）的结果显示CMSPA90-1具有良好的热稳定性。 DPPH和ABTS（+）自由基清除测定结果表明CMSPA90-1表现出自由基清除性质。否则，CMSPA90-1可以促进小鼠脾细胞的增殖和RAW264.7细胞的中性红吞噬作用，这表明CMSPA90-1可以作为潜在的功能性食品或天然药物之一进行研究和发展。

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  • 来源
    《RSC Advances》 |2019年第12期|共10页
  • 作者

    Gao Yue; Peng Bao; Xu Yafang; Yang Jia-ning; Song Li-yan; Bi Si-xue; Chen Yiyu; Zhu Jian-hua; Wen Yao; Yu Rong-min;

  • 作者单位

    Jinan Univ Biotechnol Inst Chinese Mat Med 601 Huangpu Ave West Guangzhou 510632 Guangdong Peoples R China;

    Jinan Univ Dept Nat Prod Chem Coll Pharm Guangzhou 510632 Guangdong Peoples R China;

    Jinan Univ Dept Pharmacol Coll Pharm Guangzhou 510632 Guangdong Peoples R China;

    Jinan Univ Dept Pharmacol Coll Pharm Guangzhou 510632 Guangdong Peoples R China;

    Jinan Univ Dept Pharmacol Coll Pharm Guangzhou 510632 Guangdong Peoples R China;

    Jinan Univ Biotechnol Inst Chinese Mat Med 601 Huangpu Ave West Guangzhou 510632 Guangdong Peoples R China;

    Jinan Univ Dept Nat Prod Chem Coll Pharm Guangzhou 510632 Guangdong Peoples R China;

    Jinan Univ Dept Nat Prod Chem Coll Pharm Guangzhou 510632 Guangdong Peoples R China;

    Jinan Univ Dept Pharmacol Coll Pharm Guangzhou 510632 Guangdong Peoples R China;

    Jinan Univ Biotechnol Inst Chinese Mat Med 601 Huangpu Ave West Guangzhou 510632 Guangdong Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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