• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Innovative gold-free carbon nanotube/chitosan-based competitive immunosensor for determination of HIV-related p24 capsid protein in serum
【24h】

Innovative gold-free carbon nanotube/chitosan-based competitive immunosensor for determination of HIV-related p24 capsid protein in serum

机译：基于创新的无氧碳纳米管/壳聚糖的竞争免疫传感器，用于测定血清中HIV相关的P24胶囊蛋白

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In the past decade, the need for simple, rapid, sensitive, specific and inexpensive screening methods for diagnosis of HIV infection has led to a focus on the HIV1-related capsid protein p24. In this work, the first competitive electrochemical immunosensor for the detection of p24 in untreated human serum was developed as a simple, easy-to-use and promising tool for serum screening for early diagnosis of HIV infection. The immunodevice was implemented on disposable gold-free single-walled carbon nanotube-functionalized screen-printed electrodes. The competitive sensor is based on the immobilization of the target protein on the electrode surface using a chitosan/glutaraldehyde crosslinking system, able to ensure, under mild conditions, a robust immobilization and a proper exposition of p24 for interaction with a mouse anti-p24 IgG1. The immunosensor setup as well as the assay's experimental conditions were then optimized, achieving a wide linear detection range of 10 pM to 1 nM, with a low detection limit of 2 pM in human serum. The good performance, also in terms of selectivity, trueness and precision, coupled with the advantages of an easy preparation compared to other methods requiring very complex conjugation procedures between bioreceptors and expensive nanostructures, makes the immunosensor a powerful diagnostic tool, valuable for implementation of large-scale screening programs for early diagnosis of seropositivity.
机译：在过去十年中，需要对诊断HIV感染的简单，快速，敏感，具体和廉价筛选方法导致了对HIV1相关衣壳蛋白P24的关注。在这项工作中，开发了一种用于检测未处理的人血清P24的竞争电化学免疫传感器是一种简单，易于使用和有希望的血清筛选，用于早期诊断HIV感染。免疫检测在一次性金无壁碳纳米管官能化的丝网印刷电极上实施。竞争传感器基于使用壳聚糖/戊二醛交联系统固定靶蛋白在电极表面上的固定，能够在温和的条件下保证，鲁棒固定化和P24的适当阐述与小鼠抗P24 IgG1相互作用。然后优化免疫传感器设置以及测定的实验条件，实现了10pm至1nm的宽线性检测范围，在人血清中具有低检测限。良好的性能，也是在选择性，真实和精度方面，与易于制备的优点相比，与必要的方法相比，需要具有非常复杂的生物纳米结构和昂贵的纳米结构，使免疫传感器成为强大的诊断工具，对大量实施有价值-Spale筛选计划，用于早期诊断血清阳性。

著录项

  • 来源
    《RSC Advances》 |2017年第63期|共7页
  • 作者

    Giannetto M.; Costantini M.; Mattarozzi M.; Careri M.;

  • 作者单位

    Univ Parma Dipartimento Sci Chim Vita &

    Sostenibilita Ambien Parco Area Sci 17-A I-43124 Parma Italy;

    Univ Parma Dipartimento Sci Chim Vita &

    Sostenibilita Ambien Parco Area Sci 17-A I-43124 Parma Italy;

    Univ Parma Dipartimento Sci Chim Vita &

    Sostenibilita Ambien Parco Area Sci 17-A I-43124 Parma Italy;

    Univ Parma Dipartimento Sci Chim Vita &

    Sostenibilita Ambien Parco Area Sci 17-A I-43124 Parma Italy;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号