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A real-time decoding sequencing technology-new possibility for high throughput sequencing

机译：实时解码排序技术 - 高吞吐量排序的新可能性

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摘要

A real-time decoding sequencing developed by our group offers long read length, high sequencing accuracy and high compatibility, making it have great potential in high throughput sequencing (HTS) platforms. Here, we first discuss its potential advantages in HTS in terms of read length, sequencing accuracy, and turnaround time. We then discuss its disadvantages including homopolymers and chain decoding mistakes. How to handle these two major disadvantages is also discussed with respect to resequencing and de novo sequencing. We also provide the characteristics of this technology for HTS in terms of error-correcting and discriminating SNP/deletion/insertion. Finally, the existing sequencing platforms with which this technology is compatible are discussed. This technology is not only compatible with the first-generation sequencing platform, but also the second-generation and even the third-generation sequencing platforms. It will further improve the advantages of existing sequencing platforms (read length of PGM and 454 system) and compensate some disadvantages of other next generation sequencing (NGS) platforms (sequencing accuracy of PGM sequencer). We fully hope it will provide a new promising technology for researchers and customers to extend applications of the current and upcoming platforms in almost every area in life and biomedical sciences.
机译：我们的小组开发的实时解码排序提供长读取长度，高序列精度和高兼容性，使其具有高吞吐量测序（HTS）平台具有很大的潜力。在这里，我们首先在读取长度，测序精度和周转时间方面讨论其潜在的优势。然后，我们讨论其缺点，包括均聚物和连锁错误错误。如何处理这两个主要缺点也讨论重新排列和Novo测序。在纠错和辨别SNP /删除/插入方面，我们还为HTS提供了这种技术的特征。最后，讨论了具有该技术兼容的现有测序平台。该技术不仅与第一代排序平台兼容，还兼容第二代甚至是第三代排序平台。它将进一步提高现有测序平台的优点（读取PGM和454系统的读取长度），并补偿其他下一代测序（NGS）平台的一些缺点（PGM测序器的测序精度）。我们充分希望它将为研究人员和客户提供一项新的有希望的技术，以扩展当前和即将到来的平台在生活和生物医学科学的几乎所有领域的应用。

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  • 来源
    《RSC Advances》 |2017年第64期|共11页
  • 作者

    Pu Dan; Xiao Pengfeng;

  • 作者单位

    Chongqing Univ Posts &

    Telecommun Sch Bioinformat Chongqing 400065 Peoples R China;

    Southeast Univ State Key Lab Bioelect Sch Biol Sci &

    Med Engn Nanjing 210096 Jiangsu Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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