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Aligned hydroxyapatite nano-crystal formation on a polyamide surface

机译：聚酰胺表面上对准羟基磷灰石纳米晶体形成

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摘要

Controlling the orientation of well-crystallized nano-hydroxyapatite (n-HA) remains a difficult task because of the complicated process of n-HA crystallization. In the present research, highly aligned n-HA arrays were fabricated on a polyamide matrix. The oriented n-HA crystals were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), high-resolution transmission electron microscopy (HRTEM), X-ray diffraction (XRD), X-ray photoelectric spectroscopy (XPS) and Fourier-transform infrared spectroscopy (FTIR). The mechanism of how these structures form was explored. The results show that the oriented n-HA arrays are formed on a calcium treated polyamide matrix. The diameter of the n-HA columns is about 47.41 +/- 3.02 nm. The n-HA crystals grow vertically with the substrate and the length of the crystals is 613.423 +/- 61.57 nm. Osteoblast-like MG63 cells were cultured on the nano-crystals in order to demonstrate the biocompatibility of these oriented n-HA crystals. The MTT assay suggests that the oriented n-HA crystals could promote cell proliferation. The overall results indicate the promising potential of oriented n-HA crystals for bone regeneration.
机译：由于N-HA结晶的复杂方法，控制良好结晶的纳米羟基磷灰石（N-HA）的取向仍然是困难的任务。在本研究中，在聚酰胺基质上制造高度对准的N-HA阵列。通过扫描电子显微镜（SEM），能量分散X射线光谱（EDS），高分辨率透射电子显微镜（HRTEM），X射线衍射（XRD），X射线光电光谱，以取向的N-HA晶体表征。 （XPS）和傅里叶变换红外光谱（FTIR）。探讨了这些结构形式的机制。结果表明，取向的N-HA阵列形成在钙处理的聚酰胺基质上。 N-HA柱的直径约为47.41 +/- 3.02nm。 N-HA晶体与基材垂直生长，并且晶体的长度为613.423 +/- 61.57nm。在纳米晶体上培养成骨细胞样Mg63细胞，以证明这些取向的N-Ha晶体的生物相容性。 MTT测定表明，取向的N-HA晶体可以促进细胞增殖。整体结果表明导向N-HA晶体用于骨再生的有希望的潜力。

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  • 来源
    《RSC Advances》 |2017年第68期|共7页
  • 作者

    Huang Di; Yin Meng; Lin Qiaoxia; Qin Yi; Wei Yan; Hu Yinchun; Lian Xiaojie; Guo Meiqing; Du Jingjing; Chen Weiyi;

  • 作者单位

    Taiyuan Univ Technol Res Ctr Nanobiomat &

    Regenerat Med Dept Biomed Engn Coll Mech Taiyuan 030024 Shanxi Peoples R China;

    Taiyuan Univ Technol Res Ctr Nanobiomat &

    Regenerat Med Dept Biomed Engn Coll Mech Taiyuan 030024 Shanxi Peoples R China;

    Taiyuan Univ Technol Res Ctr Nanobiomat &

    Regenerat Med Dept Biomed Engn Coll Mech Taiyuan 030024 Shanxi Peoples R China;

    Taiyuan Univ Technol Res Ctr Nanobiomat &

    Regenerat Med Dept Biomed Engn Coll Mech Taiyuan 030024 Shanxi Peoples R China;

    Taiyuan Univ Technol Res Ctr Nanobiomat &

    Regenerat Med Dept Biomed Engn Coll Mech Taiyuan 030024 Shanxi Peoples R China;

    Taiyuan Univ Technol Res Ctr Nanobiomat &

    Regenerat Med Dept Biomed Engn Coll Mech Taiyuan 030024 Shanxi Peoples R China;

    Taiyuan Univ Technol Res Ctr Nanobiomat &

    Regenerat Med Dept Biomed Engn Coll Mech Taiyuan 030024 Shanxi Peoples R China;

    Taiyuan Univ Technol Res Ctr Nanobiomat &

    Regenerat Med Dept Biomed Engn Coll Mech Taiyuan 030024 Shanxi Peoples R China;

    Taiyuan Univ Technol Res Ctr Nanobiomat &

    Regenerat Med Dept Biomed Engn Coll Mech Taiyuan 030024 Shanxi Peoples R China;

    Taiyuan Univ Technol Shanxi Key Lab Mat Strength &

    Struct Impact Inst Appl Mech &

    Biomed Engn Taiyuan 030024 Shanxi Peoples R China;

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