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Fabrication of highly stable and sensitive electrochemical sensor from hemoglobin-Au nanocomposites and its analytical applications

机译:血红蛋白-Au纳米复合材料的高稳态和敏感电化学传感器的制备及其分析应用

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摘要

To improve electron transfer, a combination of electroactive hemoglobin (Hb) and Au nanoparticles (Au NPs) was designed to obtain nanocomposites (NCs). Cyclic voltammetry and redox probe characterization showed that Hb-Au NCs incorporating the layer-by-layer technique to improve the ration of electroactive Hb, showed a better conjugated structure, lower standard entropy, and faster electron transfer. Au entrapped in Hb on the glassy carbon electrode (GCE) also exhibited a wider linear range, and excellent reproducibility and recovery for analytical applications of hydrogen peroxide, oxygen, and trichloroacetic acid. Therefore, our research could potentially become a promising alternative by combining bioactive molecules with nanoparticles, and broaden the applications of mediator-free biosensors.
机译:为了改善电子转移,设计了电活性血红蛋白(Hb)和Au纳米颗粒(Au NPS)的组合以获得纳米复合材料(NCS)。 循环伏安法和氧化还原探针表征显示,包含层逐层技术改善电活性Hb的ry的Hb-Au nc,显示出更好的共轭结构,更低的标准熵和更快的电子转移。 在玻璃碳电极(GCE)上捕获在Hb中的Au也表现出更宽的线性范围,以及用于过氧化氢,氧气和三氯乙酸的分析应用的优异再现性和恢复。 因此,我们的研究可能通过将生物活性分子与纳米颗粒组合,并扩大无介质的生物传感器的应用来成为一个有前途的替代方案。

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  • 来源
    《RSC Advances》 |2017年第68期|共7页
  • 作者单位

    Nantong Univ Inst Analyt Chem Life Sci Sch Publ Hlth Nantong 226019 Peoples R China;

    Nantong Univ Inst Analyt Chem Life Sci Sch Publ Hlth Nantong 226019 Peoples R China;

    Nantong Univ Inst Analyt Chem Life Sci Sch Publ Hlth Nantong 226019 Peoples R China;

    Nantong Univ Inst Analyt Chem Life Sci Sch Publ Hlth Nantong 226019 Peoples R China;

    Nantong Univ Inst Analyt Chem Life Sci Sch Publ Hlth Nantong 226019 Peoples R China;

    Nantong Univ Inst Analyt Chem Life Sci Sch Publ Hlth Nantong 226019 Peoples R China;

    Nantong Univ Inst Analyt Chem Life Sci Sch Publ Hlth Nantong 226019 Peoples R China;

    Nantong Univ Inst Analyt Chem Life Sci Sch Publ Hlth Nantong 226019 Peoples R China;

    Nantong Univ Inst Analyt Chem Life Sci Sch Publ Hlth Nantong 226019 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

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