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首页> 外文期刊>RSC Advances >Facile and sensitive chemiluminescence detection of H2O2 and glucose by a gravity/capillary flow and cloth-based low-cost platform
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Facile and sensitive chemiluminescence detection of H2O2 and glucose by a gravity/capillary flow and cloth-based low-cost platform

机译：通过重力/毛细管流动和布料低成本平台的H2O2和葡萄糖的容易和敏感的化学发光检测

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摘要

This paper describes the development of a cloth-based CL strategy that can quantify H2O2 or glucose in a low-cost, easy-to-use, rapid and sensitive manner. Wax screen-printing is applied to fabricate the ultralow-cost cloth-based devices, and thus the as-prepared sensors have a very low cost (about $0.01 per assay). Moreover, an inexpensive CCD camera is used to image the light from the gravity/capillary flow-triggered enzyme-catalysed oxidation of luminol by H2O2. Under optimized conditions, the developed CL platform can fulfill the determination of H2O2, with a linear range of 0.01-10 mM and a detection limit of 9.07 mM. Similarly, glucose can be detected in the range of 0.01-10 mM, with a detection limit of 9.74 mM. Additionally, the RSDs of these two assays are 3.36%, 3.66% and 2.64% for 0.01, 0.1 and 5 mM H2O2, and 3.01%, 2.80% and 3.68% for 0.1, 1 and 5 mM glucose, respectively. Moreover, the devices can maintain similar to 95% of their initial response for three weeks, and tolerate a series of interfering species at reasonable concentrations. Therefore, the proposed platform has an acceptable sensitivity, linear range, reproducibility and stability. Finally, this platform is successfully applied to the detection of H2O2 in milk and glucose in human serum.
机译：本文介绍了一种基于布的CL策略的开发，可以低成本，易于使用，快速敏感的方式量化H2O2或葡萄糖。蜡丝网印刷用于制造基于超级成本布的装置，因此制备的传感器具有非常低的成本（每次测定约0.01美元）。此外，廉价的CCD摄像机用于通过H 2 O 2将来自重力/毛细血管流动触发的荧光醇催化的光。在优化条件下，开发的CL平台可以满足H 2 O 2的测定，线性范围为0.01-10mm，检测限为9.07 mm。类似地，可以在0.01-10mm的范围内检测葡萄糖，检测限为9.74mm。另外，对于0.01,0.1和5mm H 2 O 2的3.36％，3.66％和2.64％，分别为0.01,0.1和5mm，3.01％，2.80％和3.68％，分别为0.1,1和5mm葡萄糖。此外，该装置可以保持与其初始反应的95％相似三周，并以合理的浓度耐受一系列干扰物种。因此，所提出的平台具有可接受的灵敏度，线性范围，再现性和稳定性。最后，该平台成功地应用于人血清中牛奶和葡萄糖中H2O2的检测。

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  • 来源
    《RSC Advances》 |2017年第68期|共10页
  • 作者

    Li Huijie; Wang Dan; Liu Cuiling; Liu Rui; Zhang Chunsun;

  • 作者单位

    South China Normal Univ MOE Key Lab Laser Life Sci Inst Laser Life Sci Coll Biophoton 55 Zhongshan Ave West Guangzhou 510631 Guangdong Peoples R China;

    South China Normal Univ MOE Key Lab Laser Life Sci Inst Laser Life Sci Coll Biophoton 55 Zhongshan Ave West Guangzhou 510631 Guangdong Peoples R China;

    South China Normal Univ MOE Key Lab Laser Life Sci Inst Laser Life Sci Coll Biophoton 55 Zhongshan Ave West Guangzhou 510631 Guangdong Peoples R China;

    South China Normal Univ MOE Key Lab Laser Life Sci Inst Laser Life Sci Coll Biophoton 55 Zhongshan Ave West Guangzhou 510631 Guangdong Peoples R China;

    South China Normal Univ MOE Key Lab Laser Life Sci Inst Laser Life Sci Coll Biophoton 55 Zhongshan Ave West Guangzhou 510631 Guangdong Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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