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首页> 外文期刊>RSC Advances >Influence of culture conditions on extracellular polysaccharide production and the activities of enzymes involved in the polysaccharide synthesis of Nostoc flagelliforme
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Influence of culture conditions on extracellular polysaccharide production and the activities of enzymes involved in the polysaccharide synthesis of Nostoc flagelliforme

机译：培养条件对多糖合成中腹菌鞭毛的细胞外多糖生产和酶活性的影响

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摘要

Culture conditions significantly influence extracellular polysaccharide (EPS) production of Nostoc flagelliforme, however, the key enzyme controlling EPS synthesis has not been fully explored yet. The influence of different culture conditions including light quality, carbon source and nitrogen source on EPS production of N. flagelliforme and activities of EPS synthesis enzymes was investigated. Three experimental groups produced higher amounts of EPS than the control group, including the carbon source group with 1.26 g L-1 NaHCO3, the nitrogen source group with 0 g L-1 NaNO3 and the light quality group with blue light. Activities of seven related enzymes phosphoglucose isomerase (PGI), fructose-1,6-bisphosphatase (FBPase), UDP-glucose pyrophosphorylase (UGPase), UDP-galactose-4-epimerase (UGE), UDP-glucose dehydrogenase (UGDH), phosphomannose isomerase (PMI), and phosphofructokinase (PFK) were significantly influenced by culture conditions. Partial least-squares analysis and correlation analysis methods were used to analyze the relationship between the activities of these enzymes and EPS production, and a correlation between the production of EPS and the activities of PGI, PMI, FBPase, UGDH, and UGPase was found under different culture conditions. Subsequent analysis of the transcription level of genes encoding the five enzymes showed genes pgi and fbp1 in three experimental groups were significantly up regulated. The results revealed PGI and FBPase might be important enzymes positively influencing the biosynthesis of N. flagelliforme EPS. The findings would be helpful to further understand the pathway of EPS biosynthesis aimed to improve the EPS production of N. flagelliforme.
机译：培养条件显着影响Nostoc鞭毛的细胞外多糖（EPS）产生，然而，控制EPS合成的关键酶尚未得到全面探索。研究了不同培养条件的影响，包括光质，碳源和氮源对EPS产生的N.鞭毛状和EPS合成酶的活性。三个实验组产生比对照组更多的EPS，包括碳源组，碳源基团，氮源组，具有0 g L-1纳米3的氮源组和蓝光。七种相关酶磷光葡萄糖异构酶（PGI），果糖-1,6-双磷酸酶（FBP酶），UDP-葡萄糖溶解酶（UGP酶），UDP-半乳糖-4-映异构酶（UGE），UDP-葡萄糖脱氢酶（UGDH），磷蛋白酶异构酶（PMI）和磷质子糖酶（PFK）受培养条件的显着影响。部分最小二乘分析和相关分析方法用于分析这些酶和EPS生产的活性之间的关系，并在PGI，PMI，FBP酶，UGDH和PGI，PMI，FBPAse，UGDH和UGPase之间的相关性进行相关性不同的培养条件。随后分析编码五个酶的基因的转录水平显示，三个实验组中PGI和FBP1的基因进行了监管。结果揭示了PGI和FBPase可能是重要的酶，积极影响N.鞭状EPS的生物合成。调查结果将有助于进一步了解EPS生物合成的途径，旨在改善N.Plilliforme的EPS生产。

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  • 来源
    《RSC Advances》 |2017年第71期|共10页
  • 作者

    Han Pei-pei; Yao Shun-yu; Guo Rong-jun; Yan Rong-rong; Wu Yi-kai; Shen Shi-gang; Jia Shi-ru;

  • 作者单位

    Tianjin Univ Sci &

    Technol Coll Biotechnol Minist Educ Key Lab Ind Fermentat Microbiol Tianjin 300457 Peoples R China;

    Tianjin Univ Sci &

    Technol Coll Biotechnol Minist Educ Key Lab Ind Fermentat Microbiol Tianjin 300457 Peoples R China;

    Tianjin Univ Sci &

    Technol Coll Biotechnol Minist Educ Key Lab Ind Fermentat Microbiol Tianjin 300457 Peoples R China;

    Tianjin Univ Sci &

    Technol Coll Biotechnol Minist Educ Key Lab Ind Fermentat Microbiol Tianjin 300457 Peoples R China;

    Tianjin Univ Sci &

    Technol Coll Biotechnol Minist Educ Key Lab Ind Fermentat Microbiol Tianjin 300457 Peoples R China;

    Tianjin Univ Sci &

    Technol Coll Biotechnol Minist Educ Key Lab Ind Fermentat Microbiol Tianjin 300457 Peoples R China;

    Tianjin Univ Sci &

    Technol Coll Biotechnol Minist Educ Key Lab Ind Fermentat Microbiol Tianjin 300457 Peoples R China;

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  • 中图分类 化学;
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