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Colorimetric detection of epinephrine using an optimized paper-based aptasensor

机译：使用优化的基于纸张的Aptasensor对肾上腺素的比色检测

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摘要

A simple label-free colorimetric detection of epinephrine has been demonstrated based on its interaction with aptamer functionalized gold nanoparticles. The role of different parameters such as morphology of AuNPs, aptamer concentration and salt destabilization has been thoroughly investigated to enhance the performance of the sensor in terms of sensitivity and specificity. A range of common interfering agents such as 3,4-dihydroxyphenylacetic acid (DOPAC), tryptophan, dopamine, ascorbic acid etc. showed very little/no aggregation in the solution which indicates the specificity of the sensor. The sensor was then tested on a hydrophobic paper substrate for the development of a cheap, portable and point of care diagnostic tool. A visible change in colour from red to blue was observed in the solution with increasing concentration of epinephrine. The present approach gave a detection limit of 0.9 nM using UV-visible spectroscopy which is by far the lowest detection limit of epinephrine using any colorimetric approach.
机译：基于其与适体官能化金纳米颗粒的相互作用证明了对肾上腺素的无标记比色度检测。已经彻底研究了不同参数的作用，例如AuNP，适体浓度和盐稳定化的形态，以提高传感器在敏感性和特异性方面的性能。一系列常见的干扰剂如3,4-二羟基苯基乙酸（DOPAC），色氨酸，多巴胺，抗坏血酸等在溶液中显示出非常小/没有聚集，表明传感器的特异性。然后将传感器在疏水纸基材上进行测试，以开发廉价，便携和护理点的诊断工具。在溶液中观察到肾上腺素浓度升高的溶液中从红色到蓝色的颜色变化。本方法使用UV可见光谱进行0.9nm的检出限，该光谱法通过使用任何比色方法来迄今为止肾上腺素的最低检测限。

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  • 来源
    《RSC Advances》 |2017年第77期|共11页
  • 作者

    Saraf Nileshi; Bosak Alexander; Willenberg Alicia; Das Soumen; Willenberg Bradley Jay; Seal Sudipta;

  • 作者单位

    Univ Cent Florida Dept Mat Sci &

    Engn Adv Mat Proc &

    Anal Ctr Orlando FL 32816 USA;

    Univ Cent Florida Coll Med Dept Internal Med Orlando FL 32816 USA;

    Univ Cent Florida Coll Med Dept Internal Med Orlando FL 32816 USA;

    Univ Cent Florida Dept Mat Sci &

    Engn Adv Mat Proc &

    Anal Ctr Orlando FL 32816 USA;

    Univ Cent Florida Coll Med Dept Internal Med Orlando FL 32816 USA;

    Univ Cent Florida Dept Mat Sci &

    Engn Adv Mat Proc &

    Anal Ctr Orlando FL 32816 USA;

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  • 正文语种 eng
  • 中图分类 化学;
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