Real-time visualization of cardiac cell beating behaviour on polymer diffraction gratings

Gibbons A.; Lang O.; Kojima Y.; Ito M.; Ono K.; Tanaka K.; Sivaniah E.

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Real-time visualization of cardiac cell beating behaviour on polymer diffraction gratings

机译：高分子衍射光栅对心电池搏动行为的实时可视化

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摘要

Cardiotoxicity is one of the major adverse effects of pharmaceuticals and new methods are sought for measuring cell properties with high-throughput. Here we report a flexible diffraction grating device fabricated from polydimethylsiloxane coated with platinum to make optically responsive cell culturing substrates. Cardiomyocytes from neonatal Sprague-Dawley rats were isolated and seeded upon the surface of the PDMS diffraction grating using fibronectin as the binding protein. On this substrate the cells formed a confluent layer and continued beating synchronously. By measuring the optical change of the PDMS substrate, the beating of the confluent layer could be monitored. Epinephrine was introduced to the cell environment and the change in beating frequency was measured and visualized. This flexible diffraction grating substrate provides a new way for remotely measuring cell beating properties during high-throughput testing and within miniaturized or confined device architectures.

机译：心脏毒性是药物的主要不良反应之一，并寻求具有高通量的细胞性能的新方法。在这里，我们报告了一种柔性衍射光栅装置，由涂有铂的聚二甲基硅氧烷制成，以制备光学响应性细胞培养基材。使用纤连蛋白作为结合蛋白，分离出新生儿Sprague-Dawley大鼠的心肌细胞和接种PDMS衍射光栅的表面。在该基材上，电池形成汇合层并继续跳动。通过测量PDMS基板的光学变化，可以监测汇合层的跳动。向细胞环境引入肾上腺素，测量和可视化跳动频率的变化。该柔性衍射光栅基板提供了一种新的方式，用于在高通量测试期间远程测量电池搏动性能，以及在小型化或限制装置架构内。

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《RSC Advances》 |2017年第81期|共9页
作者
Gibbons A.; Lang O.; Kojima Y.; Ito M.; Ono K.; Tanaka K.; Sivaniah E.;
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作者单位

Kyoto Univ Inst Integrated Cell Mat Sci Kyoto Japan;

Semmelweis Univ Chemotaxis Res Grp Dept Genet Cell &

Immunobiol Budapest Hungary;

Kyoto Univ Inst Integrated Cell Mat Sci Kyoto Japan;

Kyoto Univ Inst Integrated Cell Mat Sci Kyoto Japan;

Kyoto Univ Grad Sch Med Dept Cardiovasc Med Kyoto Japan;

Kyoto Univ Inst Integrated Cell Mat Sci Kyoto Japan;

Kyoto Univ Inst Integrated Cell Mat Sci Kyoto Japan;

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正文语种 eng
中图分类化学;
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1. Real-time visualization of cardiac cell beating behaviour on polymer diffraction gratings [J] . Gibbons A., Lang O., Kojima Y., RSC Advances . 2017,第81期

机译：高分子衍射光栅对心电池搏动行为的实时可视化
2. Real-time visualization of cardiac cell beating behaviour on polymer diffraction gratings [J] . A. Gibbons, O. Lang, Y. Kojima, RSC Advances . 2017,第81期

机译：在聚合物衍射光栅上实时显示心脏细胞跳动行为
3. Real-time visualization of cardiac cell beating behaviour on polymer diffraction gratings [J] . A. Gibbons, O. Lang, Y. Kojima, RSC Advances . 2017,第81期

机译：在聚合物衍射光栅上实时显示心脏细胞跳动行为
4. Metal/Polymer Back Reflectors with Diffraction Gratings for Light Trapping in III-V Solar Cells [C] . Timo Aho, Mircea Guina, Farid Elsehrawy, World Conference on Photovoltaic Energy Conversion;European Photovoltaic Solar Energy Conference;Photovoltaic Science and Engineering Conference;IEEE Photovoltaic Specialist Conference . 2018

机译：具有衍射光栅的金属/聚合物背反射镜，用于III-V太阳能电池的光阱
5. Characterization of the size-quantized electronic and optical properties of cadmium selenide nanocrystals for applications in photocatalysis, solar cells and diffraction gratings [D] . Shallcross, Richard Clayton 2009

机译：表征硒化镉纳米晶体的尺寸量子化的电子和光学性质，用于光催化，太阳能电池和衍射光栅
6. Real-time 3D visualization of cellular rearrangements during cardiac valve formation [O] . Jenny Pestel, Radhan Ramadass, Sebastien Gauvrit, -1

机译：心脏瓣膜形成过程中细胞重排的实时3D可视化
7. Real-time visualization of cardiac cell beating behaviour on polymer diffraction gratings [O] . A. Gibbons, O. Lang, Y. Kojima, 2017

机译：高分子衍射光栅对心电池搏动行为的实时可视化

Real-time visualization of cardiac cell beating behaviour on polymer diffraction gratings

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