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首页> 外文期刊>RSC Advances >Effect of PET graft coated with silk fibroin via EDC/NHS crosslink on graft-bone healing in ACL reconstruction
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Effect of PET graft coated with silk fibroin via EDC/NHS crosslink on graft-bone healing in ACL reconstruction

机译：通过EDC / NHS交联在ACL重建中涂布丝素蛋白丝纤维蛋白涂覆丝素蛋白的影响

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摘要

Anterior cruciate ligament (ACL) reconstruction under arthroscopy using (PET) artificial ligaments helps to return to sport earlier compared with using autografts and allografts, but defective biocompatibility of PET worries surgeons. The purpose of this study is to investigate whether silk fibroin coating on PET via EDC/NHS crosslink could promote the compatibility of PET and enhance graft-bone healing. SF was immobilized on the surface of PET ligaments via EDC/NHS crosslinking method. Changes in the surface properties were characterized by scanning electron microscope (SEM), water contact angle measurement, and Fourier transform infrared spectra (FTIR). SF coating enhanced the hydrophilia of PET. In vitro studies by culturing BMSCs demonstrated that SF coating could improve BMSCs proliferation and increase the expression level of BMP-2, OCN, collagen I. Rabbit ACL reconstruction model was applied to observe the graft-bone healing process in vivo. The histological results proved that SF coating promoted graft-bone healing. However, the histological improvement was not translated into significant amelioration in biomechanical results and micro-CT analysis. In conclusion, SF coating via EDC/NHS crosslink improved the graft-bone healing of PET ligaments within the bone tunnel, which might positively influence the outcome of ACL reconstruction.
机译：使用（PET）人工韧带的关节镜下的前十字韧带（ACL）重建有助于使用自体移植物和同种异体移植物更早返回运动，但宠物担心外科医生的生物相容性有缺陷。本研究的目的是探讨通过EDC / NHS Crosslink的PET丝素丝素涂层是否可以促进PET的相容性并增强接枝骨愈合。通过EDC / NHS交联方法将SF固定在PET韧带表面上。通过扫描电子显微镜（SEM），水接触角测量和傅里叶变换红外光谱（FTIR），表征了表面性质的变化。 SF涂层增强了PET的疏水性。通过培养BMSCs的体外研究证明，SF涂层可以改善BMSCs增殖并增加BMP-2，OCN，胶原I.兔ACL重建模型的表达水平被应用于观察体内移植物 - 骨愈合过程。组织学结果证明了SF涂层促进了移植物 - 骨愈合。然而，组织学改善未转化为生物力学结果和微型CT分析的显着改善。总之，通过EDC / NHS交联的SF涂层改善了骨隧道内PET韧带的移植物 - 骨愈合，这可能会对ACL重建的结果产生积极影响。

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  • 来源
    《RSC Advances》 |2017年第81期|共10页
  • 作者

    Ai Chengchong; Cai Jiangyu; Zhu Jun; Zhou Juan; Jiang Jia; Chen Shiyi;

  • 作者单位

    Fudan Univ Huashan Hosp Dept Sports Med 12 Middle Wulumuqi Rd Shanghai 200040 Peoples R China;

    Fudan Univ Huashan Hosp Dept Sports Med 12 Middle Wulumuqi Rd Shanghai 200040 Peoples R China;

    Natl Engn Res Ctr Nanotechnol Shanghai 200241 Peoples R China;

    Natl Engn Res Ctr Nanotechnol Shanghai 200241 Peoples R China;

    Fudan Univ Huashan Hosp Dept Sports Med 12 Middle Wulumuqi Rd Shanghai 200040 Peoples R China;

    Fudan Univ Huashan Hosp Dept Sports Med 12 Middle Wulumuqi Rd Shanghai 200040 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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