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首页> 外文期刊>RSC Advances >Acetylcholinesterase biosensor based on electrochemically inducing 3D graphene oxide network/multi-walled carbon nanotube composites for detection of pesticides
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Acetylcholinesterase biosensor based on electrochemically inducing 3D graphene oxide network/multi-walled carbon nanotube composites for detection of pesticides

机译：基于电化学诱导的3D石墨烯氧化物网络/多壁碳纳米管复合材料检测农药的乙酰胆碱酯酶生物传感器

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摘要

A sensitive electrochemical biosensor for determining organophosphates (OPs) and carbamate pesticides has been achieved by immobilizing acetylcholinesterase (AChE) on electrochemically inducing 3D graphene oxide network/multi-walled carbon nanotube composites (e-GON-MWCNTs). The nanocomposites of e-GON-MWCNTs can provide a favorable environment for the immobilized AChE and improve the electron transfer speed between the analyte and electrode surface. The fabricated AChE biosensors show a favorable affinity to acetylthiocholine chloride (ATCl) with a Michaelis-Menten constant of 0.43 mmol L-1. In the optimal conditions, the biosensor exhibits a linear range of 0.03-0.81 ng mL(-1) for detecting carbofuran, and two linear ranges of 0.05(-1) ng mL(-1) and 1-104 ng mL(-1) for detecting paraoxon. Furthermore, the detection limits for carbofuran and paraoxon can reach 0.015 and 0.025 ng mL(-1), respectively. The AChE biosensor exhibits good reproducibility and high stability, which demonstrates good efficiency in real sample analysis.
机译：通过固定在电化学诱导的3D石墨烯氧化物网络/多壁碳纳米管复合材料（E-GON-MWCNT）上以固定乙酰胆碱酯酶（ACHE）来实现用于确定有机磷酸酯（OPS）和氨基甲酸酯农药的敏感电化学生物传感器。 E-GON-MWCNT的纳米复合材料可以为固定的疼痛提供有利的环境，并改善分析物和电极表面之间的电子传递速度。制造的ACHE生物传感器对乙酰硫代胆碱（ATCL）具有0.43mmol L-1的Michaelis-Menten常数，表现出有利的亲和力。在最佳条件下，生物传感器表现出0.03-0.81ng ml（-1）的线性范围，用于检测碳呋喃，两个线性范围为0.05（-1）ng ml（-1）和1-104ng ml（-1 ）用于检测律师。此外，碳呋喃和律素的检测限分别可以达到0.015和0.025ng ml（-1）。 ACHE生物传感器表现出良好的再现性和高稳定性，这表明了实际样品分析中的良好效率。

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  • 来源
    《RSC Advances》 |2017年第84期|共8页
  • 作者

    Li Yanping; Zhao Ruixia; Shi Lingyun; Han Gaoyi; Xiao Yaoming;

  • 作者单位

    Shanxi Univ Inst Mol Sci Key Lab Mat Energy Convers &

    Storage Shanxi Prov Taiyuan 030006 Shanxi Peoples R China;

    Shanxi Univ Inst Mol Sci Key Lab Mat Energy Convers &

    Storage Shanxi Prov Taiyuan 030006 Shanxi Peoples R China;

    Shanxi Univ Inst Mol Sci Key Lab Mat Energy Convers &

    Storage Shanxi Prov Taiyuan 030006 Shanxi Peoples R China;

    Shanxi Univ Inst Mol Sci Key Lab Mat Energy Convers &

    Storage Shanxi Prov Taiyuan 030006 Shanxi Peoples R China;

    Shanxi Univ Inst Mol Sci Key Lab Mat Energy Convers &

    Storage Shanxi Prov Taiyuan 030006 Shanxi Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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