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首页> 外文期刊>RSC Advances >In vitro and in vivo studies of a gelatin/carboxymethyl chitosan/LAPONITE (R) composite scaffold for bone tissue engineering
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In vitro and in vivo studies of a gelatin/carboxymethyl chitosan/LAPONITE (R) composite scaffold for bone tissue engineering

机译：在体外和体内测定的明胶/羧甲基壳聚糖/丙酮（R）复合支架用于骨组织工程

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摘要

In the present study, we fabricated a biocomposite scaffold composed of carboxymethyl chitosan (CMC), gelatin and LAPONITE (R) (Lap) nanoparticles via freeze-drying and investigated its potential use in bone tissue engineering. The prepared gelatin/carboxymethyl chitosan (GC) scaffolds and laponite-incorporated scaffolds were characterized by scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR) analyses. The swelling and biodegradation were also investigated. In vitro assays such as cell attachment and proliferation, osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (rBMCSs) grown on those scaffolds and in vivo cranial bone defect assays were further carried out. We found that our prepared scaffolds had a porous architecture, and the increased Lap content resulted in improved mechanical strength, whereas the swelling ratio and degradation rate decreased. In vitro cell proliferation and live cell staining experiments demonstrated that the addition of Lap (5 and 10 wt% relative to gelatin, GC-Lap5% and GC-Lap10% respectively) would facilitate cell proliferation, but caused an inhibition effect at 15% of Lap content (GC-Lap15%). Furthermore, GC-Lap10% induced a higher degree of osteogenic differentiation of rBMSCs compared with the GC scaffold and GC-Lap5% scaffold. More importantly, in vivo cranial defect experiments revealed that the addition of Lap into the GC scaffold promoted bone regeneration. These findings indicate that a composite scaffold with Lap incorporation is a promising material for bone tissue engineering.
机译：在本研究中，我们制造羧甲基壳聚糖（CMC），明胶和LAPONITE（R）（LAP）的纳米颗粒通过冷冻干燥组成的生物复合材料的支架，并研究其在骨组织工程的潜在用途。将制备的明胶/羧甲基壳聚糖通过扫描电子显微镜（SEM）（GC）支架和合成锂皂石并入支架，其特征在于，和傅里叶变换红外光谱（FTIR）分析。肿胀和生物降解进行了研究。在体外测定，例如细胞附着和增殖，大鼠骨的成骨分化的骨髓衍生的间充质干上生长的那些支架和体内颅骨缺损测定法进一步进行细胞（rBMCSs）。我们发现，我们的准备支架具有一个多孔结构，并且增加的搭接含量导致改善的机械强度，而溶胀比和降解速率降低。在体外细胞增殖和证明立（5和分别相对于明胶，GC-Lap5％和GC-Lap10％10％（重量））的添加会促进细胞增殖的活细胞染色实验，但以15％造成的抑制效果腊含量（GC-Lap15％）。此外，GC-Lap10％诱导更高程度rBMSCs的成骨分化的与GC支架和GC-Lap5％支架进行比较。更重要的是，在体内颅骨缺损实验表明，加入立入GC支架促进骨再生。这些结果表明，与立掺入复合支架是骨组织工程有前途的材料。

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  • 来源
    《RSC Advances》 |2017年第85期|共11页
  • 作者

    Li Tao; Liu Zhonglong; Xiao Ming; Yang Zezheng; Liu Zhiyuan; Zhou Xiaojun; Wang Jinwu;

  • 作者单位

    Shanghai Jiao Tong Univ Sch Med Shanghai Key Lab Orthopaed Implant Dept Orthopaed Surg Shanghai Peoples Hosp 9 Room 701 3 Bldg 639 Zhizaoju Rd Shanghai 200011 Peoples R China;

    Shanghai Jiao Tong Univ Sch Med Shanghai Key Lab Orthopaed Implant Dept Orthopaed Surg Shanghai Peoples Hosp 9 Room 701 3 Bldg 639 Zhizaoju Rd Shanghai 200011 Peoples R China;

    Shanghai Jiao Tong Univ Sch Med Shanghai Key Lab Orthopaed Implant Dept Orthopaed Surg Shanghai Peoples Hosp 9 Room 701 3 Bldg 639 Zhizaoju Rd Shanghai 200011 Peoples R China;

    Shanghai Jiao Tong Univ Sch Med Shanghai Key Lab Orthopaed Implant Dept Orthopaed Surg Shanghai Peoples Hosp 9 Room 701 3 Bldg 639 Zhizaoju Rd Shanghai 200011 Peoples R China;

    Shanghai Jiao Tong Univ Sch Med Shanghai Key Lab Orthopaed Implant Dept Orthopaed Surg Shanghai Peoples Hosp 9 Room 701 3 Bldg 639 Zhizaoju Rd Shanghai 200011 Peoples R China;

    Shanghai Jiao Tong Univ Sch Med Shanghai Key Lab Orthopaed Implant Dept Orthopaed Surg Shanghai Peoples Hosp 9 Room 701 3 Bldg 639 Zhizaoju Rd Shanghai 200011 Peoples R China;

    Shanghai Jiao Tong Univ Sch Med Shanghai Key Lab Orthopaed Implant Dept Orthopaed Surg Shanghai Peoples Hosp 9 Room 701 3 Bldg 639 Zhizaoju Rd Shanghai 200011 Peoples R China;

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