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An on-off-on gold nanocluster-based fluorescent probe for sensitive detection of organophosphorus pesticides

机译：基于ON-OFF的金纳米光栅荧光探针，用于敏感性检测有机磷农药

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摘要

In this study, a highly sensitive fluorescent probe based on bovine serum protein-protected gold nanoclusters (BSA-AuNCs) was developed for the determination of organophosphorus pesticides (OPs). At first, BSA-AuNCs were prepared using a photo stable fluorescent substrate. Acetylcholinesterase (AChE) catalyzes the hydrolysis of acetylthiocholine iodide (ATCI) to produce thiocholine (TCh), and the sulfhydryl groups (SH-) on TCh form Au-S bonds with BSA-AuNCs; thus, the fluorescence of BSA-AuNCs weakens. On the other hand, OPs can inhibit the activity of AChE, thus preventing the generation of TCh, and the fluorescence recovery of BSA-AuNCs occurs. Under optimized experimental conditions, parathion-methyl (PM) was detected in the concentration range of 0.33-6.67 ng mL(-1) with a detection limit of 0.14 ng mL(-1) (S/N = 3), which is much lower than the maximum residue limits reported in the European Union pesticides database as well as that defined by the U.S. Department Agriculture. Moreover, the assay demonstrated highly sensitive applications in the quantitative determination of OPs in water and food samples.
机译：在该研究中，开发了基于牛血清蛋白质保护的金纳米纳米蛋白（BSA-auncs）的高敏感的荧光探针用于测定有机磷农药（OPS）。首先，使用光稳定的荧光基板制备BSA-aunc。乙酰胆碱酯酶（疼痛）催化乙酰硫代胆碱碘化物（ATCI）的水解，以产生硫代胆碱（TCH），以及TCH上的巯基（SH-）与BSA-auncs形成AU-S键;因此，BSA-Auncs的荧光削弱了。另一方面，OPS可以抑制疼痛的活性，从而防止产生TCH，并且发生BSA-auncs的荧光恢复。在优化的实验条件下，在0.33-6.67 ng ml（-1）的浓度范围内检测硫磷 - 甲基（PM），检测限为0.14 ng ml（-1）（s / n = 3），这是多少低于欧洲联盟杀虫剂数据库中报告的最大残留限制以及由美国部门农业定义的最大残留限制。此外，测定在水和食物样品中的定量测定中表现出高度敏感的应用。

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  • 来源
    《RSC Advances》 |2017年第87期|共7页
  • 作者

    Luo Q. J.; Li Z. G.; Lai J. H.; Li F. Q.; Qiu P.; Wang X. L.;

  • 作者单位

    Nanchang Univ Dept Chem Nanchang 330031 Jiangxi Peoples R China;

    Jiangxi Med Device Testing Ctr Nanchang 330047 Jiangxi Peoples R China;

    Jiangxi Med Device Testing Ctr Nanchang 330047 Jiangxi Peoples R China;

    East China Univ Technol Dept Chem Nanchang 330013 Jiangxi Peoples R China;

    Nanchang Univ Dept Chem Nanchang 330031 Jiangxi Peoples R China;

    Nanchang Univ Inst Translat Med Nanchang 330088 Jiangxi Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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