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Highly sensitive and selective fluorescence detection of Hg2+ based on turn-on aptamer DNA silver nanoclusters

机译：基于开启Aptamer DNA银纳米团簇的高敏感和选择性荧光检测HG2 +

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摘要

A novel turn-on Hg2+ sensor was constructed based on fluorescent C-Hg2+-aptamer DNA-stabilized Ag nanoclusters (DNA-AgNCs), and was used to determine the concentration of Hg2+ over the range 218 nM with a detection limit as low as 0.25 nM. The sensing assay relied on a target-induced conformational transition of Hg2+-aptamer DNA. The conformational change of Hg2+-aptamer DNA from a single strand to a hairpin DNA due to the formation of a T-Hg2+-T complex in the presence of Hg2+ made the two darkish DNA-AgNCs approach each other closely and then enhanced the fluorescence of the AgNCs, which enabled the sensitive and specific detection of Hg2+. The proposed sensor was found to be easy to use, and allowed for the sensitive, selective, and turn-on detection of Hg2+. Furthermore, this approach has also been successfully applied to the detection of Hg2+ in real water samples, so this sensor may find application in monitoring Hg2+ in environmental samples.
机译：基于荧光C-HG2 + -Aptamer DNA稳定的Ag纳米能器（DNA-AGNC）构建了一种新型开启HG2 +传感器，并用于测定218nm范围内Hg2 +的浓度，检测限为0.25 纳米。 感测测定依赖于HG2 + -Aptamer DNA的目标诱导的构象转变。 Hg2 + -aptamer DNA从单链到发夹DNA的构象变化由于在HG2 +的存在下形成T-HG2 + -T络合物，使得两种黑暗的DNA-AGNC彼此紧密地接近，然后增强了荧光 AGNCS，它使HG2 +的敏感和特异性检测。 发现所提出的传感器易于使用，并允许敏感，选择性和接通HG2 +的检测。 此外，这种方法也已成功应用于实际水样中的HG2 +的检测，因此该传感器可以在环境样本中监测HG2 +的应用。

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  • 来源
    《RSC Advances》 |2017年第89期|共7页
  • 作者

    Zhang Baozhu; Wei Chunying;

  • 作者单位

    Shanxi Univ Inst Mol Sci Minist Educ Key Lab Chem Biol &

    Mol Engn Taiyuan 030006 Shanxi Peoples R China;

    Shanxi Univ Inst Mol Sci Minist Educ Key Lab Chem Biol &

    Mol Engn Taiyuan 030006 Shanxi Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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