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首页> 外文期刊>Conservation Genetics Resources >Characterization of 13 microsatellite loci for the tope shark, Galeorhinus galeus, discovered with next-generation sequencing and their utility for eastern Pacific smooth-hound sharks (Mustelus)
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Characterization of 13 microsatellite loci for the tope shark, Galeorhinus galeus, discovered with next-generation sequencing and their utility for eastern Pacific smooth-hound sharks (Mustelus)

机译：下一代测序发现的面向鲨的13个微卫星基因座的特征及其在东太平洋平滑猎犬鲨（Mustelus）中的用途

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摘要

The tope shark, Galeorhinus galeus, is a commercially important member of the Triakidae that has been exploited globally for the past 80 years. Here we describe 13 microsatellite loci for G. galeus discovered by next-generation sequencing (Roche 454 pyrosequencing) and their utility for eastern Pacific smooth-hound sharks (Mustelus). These loci were polymorphic (3-12 alleles) with observed heterozygosity between 0.11 and 0.86 and expected heterozygosity between 0.24 and 0.87. Several loci (7 of 13) amplified consistently for Mustelus californicus and M. henlei. These loci are the first to be characterized explicitly for G. galeus and should be useful in the investigation of population structure of this vulnerable elasmobranch.
机译：鲨鱼Galeorhinus galeus是Triakidae的商业重要成员，在过去的80年中，Triakidae在全球得到了广泛的利用。在这里，我们描述了由下一代测序（Roche 454焦磷酸测序）发现的G. galeus的13个微卫星基因座，及其对东太平洋平滑猎犬（Mustelus）的效用。这些基因座是多态性的（3-12个等位基因），观察到的杂合度在0.11和0.86之间，预期的杂合度在0.24和0.87之间。几个位点（13个中的7个）持续一致地扩增出了鼬芥和亨利氏菌。这些基因座是第一个明确针对G. galeus的特征，应在调查该易感弹性支流的种群结构中有用。

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