Bufo MHC class II loci with conserved introns flanking exon 2: cross-species amplification with common primers

Inga Zeisset; Trevor J. C. Beebee

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Bufo MHC class II loci with conserved introns flanking exon 2: cross-species amplification with common primers

机译：Bufo MHC II类基因座，带有保守的内含子，位于第2外显子侧翼2：使用通用引物进行跨物种扩增

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We characterized the complete second exon with adjacent introns of MHC class II beta genes in the anurans Bufo calamita, B. bufo and B. viridis. Introns were remarkably conserved among species. There were at least two loci (A and B), which we amplified with different forward primers but identical reverse primers. Locus A had low diversity whereas locus B exhibited 42 alleles in B. calamita and 14 in B. bufo. We detected evidence of positive selection at antigen binding sites in both these species anddemonstrated expression of locus B in B. calamita.

机译：我们用无头蟾蜍，B. bufo和B. viridis中的MHC II类β基因的相邻内含子表征了完整的第二个外显子。内含子在物种之间非常保守。至少有两个基因座（A和B），我们用不同的正向引物但相同的反向引物进行了扩增。基因座A的多样性较低，而基因座B在calamita芽孢杆菌中有42个等位基因，而在B. bufo芽孢杆菌中有14个等位基因。我们在这两个物种的抗原结合位点检测到阳性选择的证据，并在B. calamita中证实了基因座B的表达。

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《Conservation Genetics Resources》 |2013年第1期|共3页
作者
Inga Zeisset; Trevor J. C. Beebee;
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正文语种 eng
中图分类普通生物学;
关键词
MHC; Beta chain; Bufo calamita; Bufo bufo; Bufo viridis;

机译：MHC;Beta链;中华大蟾蜍;中华大蟾蜍;中华大蟾蜍;

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1. Bufo MHC class II loci with conserved introns flanking exon 2: cross-species amplification with common primers [J] . Inga Zeisset, Trevor J. C. Beebee Conservation Genetics Resources . 2013,第1期

机译：Bufo MHC II类基因座，带有保守的内含子，位于第2外显子侧翼2：使用通用引物进行跨物种扩增
2. Polymerase chain reaction primers for microsatellite loci in the Common Toad Bufo bufo [J] . Edward G. Brede, Graham Rowe, Jan Trojanowski, Molecular ecology notes . 2001,第4期

机译：蟾蜍蟾蜍中微卫星基因座的聚合酶链反应引物
3. Polymorphism across an exon-intron boundary in an avian Mhc class IIB gene [J] . Garrigan D., Edwards SV. Molecular biology and evolution . 1999,第11期

机译：禽类Mhc IIB类基因中外显子-内含子边界的多态性
4. Investigating the Intrinsic Differences in Flank Regions of Exon-Intron Junction Sites [C] . Sing-Wu Liou, Yin-Fu Huang International Conference on Biomedical Engineering and Informatics . 2008

机译：调查外显子内连接位点的侧枝区内在差异
5. Defining the mechanism of MHC class II peptide editing by HLA-DM: I. HLA-DM recognizes the flexible conformation of major histocompatibility complex class II. II. Mapping the interaction surface of DR1 with DM by superantigen blocking. III. Determinati [D] . Chou, Chih-Ling. 2003

机译：定义HLA-DM编辑MHC II类肽的机制：I. HLA-DM识别II类主要组织相容性复合物的柔性构象。二。通过超抗原阻断作用绘制DR1与DM的相互作用表面。三，确定性
6. NetMHCIIpan-3.0 a common pan-specific MHC class II prediction method including all three human MHC class II isotypes HLA-DR HLA-DP and HLA-DQ [O] . Edita Karosiene, Michael Rasmussen, Thomas Blicher, -1

机译：NetMHCIIpan-3.0一种常见的泛特异性MHC II类预测方法包括所有三种人类MHC II类同种型HLA-DRHLA-DP和HLA-DQ
7. Primers for the amplification of the MHC IIβ chain exon 2 in the Atlantic goliath grouper (Epinephelus itajara) [O] . Gláucia C. Silva-Oliveira, Anete B. C. Silva, Fabian Blanchard, 2014

机译：在大西洋戈尔基岛石斑鱼（Epinephelus Itajara）中扩增MHCIIβ链外显子2的引物

Bufo MHC class II loci with conserved introns flanking exon 2: cross-species amplification with common primers

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