首页> 外文期刊>Optics Communications: A Journal Devoted to the Rapid Publication of Short Contributions in the Field of Optics and Interaction of Light with Matter >Differential holographic reconstruction for lensless in-line holographic microscope with ultra-broadband light source illumination
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Differential holographic reconstruction for lensless in-line holographic microscope with ultra-broadband light source illumination

机译:具有超宽带光源照明的无透镜在线全息显微镜的差分全息重建

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摘要

We propose a differential holographic reconstruction (DHR) method for lensless in-line holographic microscope (LIHM) with ultra-broadband light source illumination. Using the DHR method, the deterioration of imaging resolution and contrast because of insufficient temporal coherence can be noticeably compensated. In our LIHM setup, the sample was illuminated by an ultra-broadband light source without any spectral fillers, and the in-line hologram was recorded by a CMOS imaging sensor located behind the sample. The enhanced-resolution sample image was achieved by subtracting two low-resolution sample images, which were obtained by back-propagating the hologram numerically to the sample plane and another plane with slightly different back-propagation distance. The DHR method was studied theoretically, and the resolution enhancement capability was verified by both numerical simulations and imaging experiments. The U.S. air force target and a lily anther as bio-sample were used in the imaging experiments. The results showed that the proposed DHR method achieved better resolution with ultra-broadband light sources such as sunlight and incandescent light sources, which could reduce the cost for compact imaging systems.
机译:我们提出了具有超宽带光源照明的无透镜在线全息显微镜(LIHM)的差分全息重建(DHR)方法。使用DHR方法,可以明显补偿成像分辨率和对比度的劣化。可以明显补偿。在我们的LiHM设置中,通过没有任何光谱填充物的超宽带光源照射样品,并通过位于样品后面的CMOS成像传感器记录在线全息图。通过减去两个低分辨率样本图像来实现增强分辨率样本图像,该低分辨率采样图像通过数字地向样本平面和另一个具有略微不同的背部传播距离的另一个平面来获得全息图而获得。理论上研究了DHR方法,通过数值模拟和成像实验验证了分辨率提高能力。在成像实验中使用美国空军靶标和百合花药作为生物样品。结果表明,所提出的DHR方法采用阳光和白炽光源等超宽带光源实现了更好的分辨率,这可以降低紧凑型成像系统的成本。

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