High-speed label-free ultraviolet photoacoustic microscopy for histology-like imaging of unprocessed biological tissues

Li Xiufeng; Kang Lei; Zhang Yan; Wong Terence T. W.

首页> 外文期刊>Optics Letters >High-speed label-free ultraviolet photoacoustic microscopy for histology-like imaging of unprocessed biological tissues

【24h】

High-speed label-free ultraviolet photoacoustic microscopy for histology-like imaging of unprocessed biological tissues

机译：无高速标记的紫外光声学显微镜，用于组织学的成像成像的未加工生物组织

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Ultraviolet photoacoustic microscopy (UV-PAM) has recently been demonstrated as a potential imaging tool for surgical margin analysis (SMA). UV-PAM does not require staining or micrometer-thick slicing, which is inevitable in conventional histological imaging. To promote UV-PAM as a practical intraoperative diagnostic tool, the imaging speed should be improved while preserving the high-resolution imaging capability and simplistic system design. In this Letter, we developed a galvanometer mirror-based UV-PAM (GM-UV-PAM) system for high-speed histology-like imaging. By using a UV laser with a high repetition rate (55 kHz) and a one-dimensional galvanometer mirror, our GM-UV-PAM system can generate subcellular images in less than 15 min for a typical brain biopsy (5 mm x 5 mm), with a lateral resolution of similar to 1.0 mu m. The images of mouse brain slices obtained by our GM-UV-PAM system show that it can provide histological information for SMA. (C) 2020 Optical Society of America

机译：最近已被证明紫外光声显微镜（UV-PAM）作为用于外科护频分析（SMA）的潜在成像工具。 UV-PAM不需要染色或微米厚切片，这在常规组织学成像中是不可避免的。为了促进UV-PAM作为实际的术中诊断工具，应在保持高分辨率成像能力和简单系统设计的同时提高成像速度。在这封信中，我们开发了一种基于镜像镜的紫外线（GM-UV-PAM）系统，用于高速组织学类似的成像。通过使用具有高重复率（55 kHz）和一维电流计镜的UV激光器，我们的GM-UV-PAM系统可以在典型的脑活检（5mm x 5 mm）的情况下在不到15分钟内产生亚细胞图像，具有类似于1.0 mu m的横向分辨率。通过我们的GM-UV-PAM系统获得的小鼠脑切片的图像表明它可以为SMA提供组织学信息。（c）2020美国光学学会

著录项

来源
《Optics Letters》 |2020年第19期|共4页
作者
Li Xiufeng; Kang Lei; Zhang Yan; Wong Terence T. W.;
展开▼
作者单位

Hong Kong Univ Sci &

Technol Dept Chem &

Biol Engn Translat &

Adv Bioimaging Lab Hong Kong Peoples R China;

Hong Kong Univ Sci &

Technol Dept Chem &

Biol Engn Translat &

Adv Bioimaging Lab Hong Kong Peoples R China;

Hong Kong Univ Sci &

Technol Dept Chem &

Biol Engn Translat &

Adv Bioimaging Lab Hong Kong Peoples R China;

Hong Kong Univ Sci &

Technol Dept Chem &

Biol Engn Translat &

Adv Bioimaging Lab Hong Kong Peoples R China;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类计量学;光学;
关键词

相似文献

外文文献
中文文献
专利

1. High-speed label-free ultraviolet photoacoustic microscopy for histology-like imaging of unprocessed biological tissues [J] . Li Xiufeng, Kang Lei, Zhang Yan, Optics Letters . 2020,第19期

机译：无高速标记的紫外光声学显微镜，用于组织学的成像成像的未加工生物组织
2. High-resolution, high-contrast mid-infrared imaging of fresh biological samples with ultraviolet-localized photoacoustic microscopy [J] . Shi Junhui, Wong Terence T. W., He Yun, Nature photonics . 2019,第9期

机译：高分辨率，高对比度的新鲜生物样品中红外成像，具有紫外线局部光声显微镜
3. Label-free biochemical imaging of heart tissue with high-speed spontaneous Raman microscopy. [J] . Ogawa M, Harada Y, Yamaoka Y, Biochemical and Biophysical Research Communications . 2009,第2期

机译：高速自发拉曼显微镜对心脏组织的无标记生化成像。
4. High-speed Label-free Galvo Mirror Scanning-based Ultraviolet Photoacoustic Microscopy for Histological Imaging [C] . Xiufeng Li, Lei Kang, Yan Zhang, Conference on Photons Plus Ultrasound: Imaging and Sensing . 2020

机译：用于组织学成像的基于高速无标签的镀锌镜扫描的紫外光声学显微镜
5. Enabling high-speed wide-field dynamic imaging in multi-focal photoacoustic computed microscopy. [D] . Wan, Hongying. 2016

机译：在多焦点光声计算机显微镜中启用高速广域动态成像。
6. Single acquisition label-free histology-like imaging with dual-contrast photoacoustic remote sensing microscopy [O] . Benjamin Ecclestone, Deepak Dinakaran, Parsin Haji Reza 2021

机译：单一采集标签的组织学类似的成像具有双对比度光声遥感显微镜
7. High-Speed Label-Free Spectroscopic Biological Imaging Based on Stimulated Raman Scattering MicroscopyHigh-Speed Label-Free Spectroscopic Biological Imaging Based on Stimulated Raman Scattering MicroscopyHigh-Speed Label-Free Spectroscopic Biological Imaging Based on Stimulated Raman Scattering Microscopy [O] . Yasuyuki OZEKI 2017

机译：基于刺激拉曼散射显微镜基于刺激拉曼散射显微镜的刺激拉曼散射微观标记无尺寸标记的无刺激性散射微观标记生物成像的高速标记的光谱散射生物成像

High-speed label-free ultraviolet photoacoustic microscopy for histology-like imaging of unprocessed biological tissues

摘要

著录项

相似文献

相关主题

期刊订阅