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Simultaneous scattering-absorption dual-modal cell imaging in a single shot by a transmission-mode photoacoustic microscope

机译：通过透射模式光声显微镜同时散射吸收双模态细胞成像

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摘要

A microscopy scheme is proposed to simultaneously achieve optical scattering-absorption dual-contrast imaging of a transparent or semi-transparent specimen. This scheme is based on a transmission-mode photoacoustic microscope. We find that two peaks exist in the detected photoacoustic signal. One peak is caused by the optical absorption of the specimen, and the other is related to both the optical scattering and absorption of the specimen. Therefore, both the absorption and scattering information can be simultaneously extracted by analyzing the same photoacoustic signal excited by a single-shot laser pulse. After the microscope is validated by imaging a binary mixture consisting of particles with different optical properties, it successfully acquires dual images of red blood cells with different contrasts. Quantitative analysis reveals that the optical absorption and scattering properties of the specimen can be derived from the two images. The proposed dual-modal imaging method would be useful in revealing the structural and functional properties of tissues at the cell level or the clinical assessment of pathological sections. (C) 2020 Optical Society of America
机译：提出了一种显微镜方案，同时实现透明或半透明标本的光散射吸收双对比度成像。该方案基于透射模式光声显微镜。我们发现检测到的光声信号中存在两个峰值。一个峰是由样本的光学吸收引起的，另一个峰值与样品的光学散射和吸收有关。因此，通过分析由单次激光脉冲激发的相同光声信号可以同时提取吸收和散射信息。通过对具有不同光学性质组成的颗粒组成的二元混合物来验证显微镜后，它成功地以不同的对比度获得了红细胞的双重图像。定量分析表明，样品的光学吸收和散射性能可以衍生自两个图像。所提出的双模态成像方法可用于揭示细胞水平的组织的结构和功能性或病理切片的临床评估。 （c）2020美国光学学会

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  • 来源
    《Optics Letters》 |2020年第20期|共4页
  • 作者

    Wu Shiqing; Tao Chao; Zhang Xiang; Lu Feng; Liu Xiaojun;

  • 作者单位

    Nanjing Univ Dept Phys Collaborat Innovat Ctr Adv Microstruct MOE Key Lab Modern Acoust Nanjing 210093 Peoples R China;

    Nanjing Univ Dept Phys Collaborat Innovat Ctr Adv Microstruct MOE Key Lab Modern Acoust Nanjing 210093 Peoples R China;

    Nanjing Univ Dept Phys Collaborat Innovat Ctr Adv Microstruct MOE Key Lab Modern Acoust Nanjing 210093 Peoples R China;

    Nanjing Univ Posts &

    Telecommun IAM Key Lab Organ Elect &

    Informat Displays Nanjing 210023 Peoples R China;

    Nanjing Univ Dept Phys Collaborat Innovat Ctr Adv Microstruct MOE Key Lab Modern Acoust Nanjing 210093 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 计量学;光学;
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