Characterizing chromatin packing scaling in whole nuclei using interferometric microscopy

Eid Aya; Eshein Adam; Li Yue; Virk Ranya; Van Derway David; Zhang Di; Taflove Allen; Backman Vadim

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Characterizing chromatin packing scaling in whole nuclei using interferometric microscopy

机译：干涉式显微镜在整个核中表征染色质包装缩放

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Chromatin is the macromolecular assembly containing the cell's genetic information, and its architectural conformation facilitates accessibility to activation sites and thus gene expression. We have developed an analytical framework to quantify chromatin structure with spectral microscopy. Chromatin structure can be described as a mass fractal, with packing scaling D up to specific genomic length scales. Considering various system geometries, we established a model to measure D with the interferometric technique partial wave spectroscopy (PWS) and validated the analysis using finite difference time domain to simulate the PWS system. Calculations of D were consistent with ground truth electron microscopy measurements, enabling a high-throughput, label-free approach to quantifying chromatin structure in the nanometer length scale regime. (C) 2020 Optical Society of America

机译：染色质是含有细胞遗传信息的大分子组件，其建筑兼容性有助于可携带活化位点，从而促进基因表达。我们开发了一种分析框架，以通过光谱显微镜量化染色质结构。染色质结构可以描述为质量分形，填充缩放D至特定的基因组长度尺度。考虑到各种系统几何形状，我们建立了用干涉测量技术部分波谱（PWS）测量D的模型，并使用有限差分时域验证了分析来模拟PWS系统。 D的计算与地面真相电子显微镜测量一致，从而实现高通量，无标记的方法来定量纳米长度尺度制度中的染色质结构。（c）2020美国光学学会

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《Optics Letters》 |2020年第17期|共4页
作者
Eid Aya; Eshein Adam; Li Yue; Virk Ranya; Van Derway David; Zhang Di; Taflove Allen; Backman Vadim;
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Northwestern Univ Dept Biomed Engn Evanston IL 60208 USA;

Northwestern Univ Dept Biomed Engn Evanston IL 60208 USA;

Northwestern Univ Dept Biomed Engn Evanston IL 60208 USA;

Northwestern Univ Dept Biomed Engn Evanston IL 60208 USA;

Northwestern Univ Dept Biomed Engn Evanston IL 60208 USA;

Northwestern Univ Dept Biomed Engn Evanston IL 60208 USA;

Northwestern Univ Dept Elect Engn Evanston IL 60208 USA;

Northwestern Univ Dept Biomed Engn Evanston IL 60208 USA;

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原文格式 PDF
正文语种 eng
中图分类计量学;光学;
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1. Characterizing chromatin packing scaling in whole nuclei using interferometric microscopy [J] . Eid Aya, Eshein Adam, Li Yue, Optics Letters . 2020,第17期

机译：干涉式显微镜在整个核中表征染色质包装缩放
2. Structural hierarchy of chromatin in chicken erythrocyte nuclei based on small-angle neutron scattering: Fractal nature of the large-scale chromatin organization [J] . Lebedev DV, Filatov MV, Kuklin AI, Crystallography reports . 2008,第1期

机译：基于小角度中子散射的鸡红细胞核中染色质的结构层次：大规模染色质组织的分形性质
3. Characterization of chromatin domains by 3D fluorescence microscopy: An automated methodology for quantitative analysis and nuclei screening [J] . Cantaloube Sylvain, Romeo Kelly, Le Baccon Patricia, BioEssays : . 2012,第6期

机译：通过3D荧光显微镜表征染色质结构域：用于定量分析和细胞核筛选的自动化方法
4. In silico analysis of nuclei in glioblastoma using large-scale microscopy images improves prediction of treatment response [C] . Kong Jun, Cooper Lee, Moreno Carlos, 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society . 2011

机译：使用大规模显微镜图像对胶质母细胞瘤细胞核进行计算机分析，可改善对治疗反应的预测
5. Biophysical Characterization of the Dynamic Regulation of Chromatin Structure and Rheology in Human Cell Nuclei. [D] . Spagnol, Stephen T. 2015

机译：人细胞核中染色质结构和流变学动态调节的生物物理表征。
6. Chromatin freeze fracture electron microscopy: a comparative study of core particles chromatin metaphase chromosomes and nuclei. [O] . J Lepault, S Bram, J Escaig, 1980

机译：染色质冷冻断裂电子显微镜：核心颗粒染色质中期染色体和细胞核的比较研究。
7. Chromatin freeze fracture electron microscopy: a comparative study of core particles, chromatin, metaphase chromosomes, and nuclei. [O] . Lepault, J, Bram, S, Escaig, J, 1980

机译：染色质冷冻断裂电子显微镜：核心颗粒，染色质，中期染色体和细胞核的比较研究。
8. Nanometer-Scale Compositional Structure in III-V Semiconductor Heterostructures Characterized by Scanning Tunneling Microscopy; Journal of Vacuum Science Technology A [R] . Allerman, A. A. , Bi, W. G. , Biefeld, R. M. , 1998

机译：通过扫描隧道显微镜表征的III-V半导体异质结构的纳米级组成结构;真空科学技术杂志a

Characterizing chromatin packing scaling in whole nuclei using interferometric microscopy

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