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Enlarged field of view based on Schwartz modulation for light sheet fluorescence microscopy in deep tissue

机译:基于Schwartz调制在深组织中的光板荧光显微镜的施加视野

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摘要

The combination of light sheet fluorescence microscopy (LSFM) and the optical clearing method can achieve fast three-dimensional high-resolution imaging. However, there is an essential contradiction between the field of view (FoV) and spatial resolution. Also, aberration and scattering still exist after tissue clearing, which seriously limits the imaging depth of LSFM. Here we propose a Schwartz modulation method and implement it in LSFM based on a quasi-Bessel beam to enlarge the imaging FoV without sacrificing its spatial resolution. The simulation results show that the FoV of the LSFM is enlarged by a factor of 1.73 compared to the Bessel beam. The capability of extremely fast decay along the optical axis makes Schwartz modulation more tolerant for scattering, indicating potential applications for deep tissue imaging. Also, the capability of sidelobe suppression effectively decreases unnecessary fluorescence excitation and photobleaching. (C) 2020 Optical Society of America
机译:光片荧光显微镜(LSFM)和光学清洁方法的组合可以实现快速的三维高分辨率成像。 然而,在视野(FOV)和空间分辨率之间存在必要的矛盾。 此外,在组织清除后仍然存在像差和散射,这严重限制了LSFM的成像深度。 在这里,我们提出了一种基于准贝塞尔光束在LSFM中实现Schwartz调制方法,并在不牺牲其空间分辨率的情况下扩大成像FOV。 仿真结果表明,与贝塞尔光束相比,LSFM的FOV被放大1.73。 沿光轴极快衰减的能力使Schwartz调制更容易散射,表明深层组织成像的潜在应用。 而且,侧链抑制的能力有效地降低了不必要的荧光激发和光漂白。 (c)2020美国光学学会

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  • 来源
    《Optics Letters》 |2020年第17期|共4页
  • 作者单位

    Zhejiang Univ Sch Med NHC &

    CAMS Key Lab Med Neurobiol Dept Neurobiol Affiliated Hosp 2 Hangzhou 310058 Peoples R China;

    Zhejiang Univ Coll Opt Sci &

    Engn Hangzhou 310027 Peoples R China;

    Zhejiang Univ Coll Opt Sci &

    Engn Hangzhou 310027 Peoples R China;

    Zhejiang Univ Sch Med NHC &

    CAMS Key Lab Med Neurobiol Dept Neurobiol Affiliated Hosp 2 Hangzhou 310058 Peoples R China;

    Zhejiang Univ Sch Brain Sci &

    Brain Med Hangzhou 310058 Peoples R China;

    Zhejiang Univ Sch Brain Sci &

    Brain Med Hangzhou 310058 Peoples R China;

    Zhejiang Univ Sch Brain Sci &

    Brain Med Hangzhou 310058 Peoples R China;

    Zhejiang Univ Sch Med NHC &

    CAMS Key Lab Med Neurobiol Dept Neurobiol Affiliated Hosp 2 Hangzhou 310058 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 计量学;光学;
  • 关键词

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