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首页> 外文期刊>Optics Letters >Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection
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Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection

机译:通过时间门控光谱复用检测快速拉曼光谱映射高荧光样本

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摘要

We present a time-gated Raman micro-spectroscopy technique suitable for fast Raman mapping of samples eliciting large laser-induced fluorescence backgrounds. To achieve the required time resolution for effective fluorescence rejection, a picosecond pulsed laser and a single-photon avalanche diode were used. A module consisting of a spectrometer, digital micromirror device, and two prisms was used for high-resolution spectral filtering and multiplexing, which is required for a high chemical specificity and short integration times. With this instrument, we demonstrated time-gated Raman imaging of highly fluorescent samples, achieving acquisition times as short as 3 min for 40 x 40 pixel resolution images.
机译:我们提出了一种时代的拉曼微谱技术,适用于引发大型激光诱导的荧光背景的样品的快速拉曼映射。 为了实现有效荧光抑制所需的时间分辨率,使用皮秒脉冲激光和单光子雪崩二极管。 由光谱仪,数字微镜器件和两种棱镜组成的模块用于高分辨率光谱滤波和复用,这是高化学特异性和短的集成时间所必需的。 利用这种仪器,我们展示了高度荧光样本的时间门控拉曼成像,实现了40×40像素分辨率图像的短至3分钟的采集时间。

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  • 来源
    《Optics Letters》 |2018年第23期|共4页
  • 作者单位

    Univ Nottingham Sch Phys &

    Astron Nottingham NG7 2RD England;

    Univ Nottingham Sch Phys &

    Astron Nottingham NG7 2RD England;

    UKRI STFC Rutherford Appleton Lab Cent Laser Facil Res Complex Harwell Didcot OX11 0QX Oxon England;

    Univ Nottingham Sch Phys &

    Astron Nottingham NG7 2RD England;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 计量学;光学;
  • 关键词

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