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Dual-wavelength digital holographic phase and fluorescence microscopy for an optical thickness encoded suspension array

机译：双波长数字全息相和光学厚度编码悬架阵列的荧光显微镜

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摘要

A dual-wavelength digital holographic phase and fluorescence microscopy system is demonstrated as the decoding and detection platform of an optical thickness encoded suspension array. The phase imaging path is designed to decode optical thickness encoded microcarriers, and the fluorescence imaging path is used to detect the quantitative information of the bound target analytes. The encoding capacity could be more than 100. The decoding reliability of the phase imaging path is verified by a multiplexed immunoassay experiment. The ability for quantitative analysis of the fluorescence imaging path is confirmed by concentration gradient experiments. This method offers high decoding accuracy and high detection sensitivity of label signals. (C) 2018 Optical Society of America
机译：双波长数字全息相和荧光显微镜系统被证明为光学厚度编码悬架阵列的解码和检测平台。 相成像路径被设计为解码光学厚度编码的微载波，并且荧光成像路径用于检测结合目标分析物的定量信息。 编码容量可能大于100.通过多路复用的免疫测定实验验证相位成像路径的解码可靠性。 通过浓度梯度实验证实了荧光成像路径的定量分析能力。 该方法提供高解码精度和标签信号的高检测灵敏度。 （c）2018年光学学会

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  • 来源
    《Optics Letters》 |2018年第4期|共4页
  • 作者

    Shen Zhiyuan; He Yonghong; Zhang Gong; He Qinghua; Li Dongmei; Ji Yanhong;

  • 作者单位

    Tsinghua Univ Grad Sch Shenzhen Inst Opt Imaging &

    Sensing Shenzhen Key Lab Minimal Invas Med Technol Shenzhen 518055 Peoples R China;

    Tsinghua Univ Grad Sch Shenzhen Inst Opt Imaging &

    Sensing Shenzhen Key Lab Minimal Invas Med Technol Shenzhen 518055 Peoples R China;

    Tsinghua Univ Grad Sch Shenzhen Inst Opt Imaging &

    Sensing Shenzhen Key Lab Minimal Invas Med Technol Shenzhen 518055 Peoples R China;

    Tsinghua Univ Grad Sch Shenzhen Inst Opt Imaging &

    Sensing Shenzhen Key Lab Minimal Invas Med Technol Shenzhen 518055 Peoples R China;

    Tsinghua Univ Grad Sch Shenzhen Inst Opt Imaging &

    Sensing Shenzhen Key Lab Minimal Invas Med Technol Shenzhen 518055 Peoples R China;

    South China Normal Univ Sch Phys &

    Telecommun Engn Guangzhou 510006 Guangdong Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 计量学;光学;
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