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Effect of refractive index mismatch on forward-to-backward ratios in SHG imaging

机译:折射率不匹配对SHG成像中向后比率的影响

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摘要

Nonlinear optical imaging in the epi-direction is used to image subresolution features. We find that a refractive index mismatch between the object to be imaged and the background medium can change the far-field intensity image. As an example, we study second harmonic generation (SHG) microscopy where the forward-to-backward (F/B) ratio is used to quantify subresolution features. We show both theoretically and experimentally that the inhomogeneous refractive index in collagen tendon tissue creates near-field effects, which can change the F/B ratio by similar to 20%-25%, even though the effect is negligible for most of the individual fibrils in the tissue. This is caused by the sensitivity of the backward signal on phase matching conditions. (c) 2018 Optical Society of America
机译:在外延方向上的非线性光学成像用于图像亚排校度特征。 我们发现要成像的对象和背景介质之间的折射率不匹配可以改变远场强度图像。 作为示例,我们研究了第二次谐波产生(SHG)显微镜,其中前向后(F / B)比率用于量化亚排校度特征。 理论上和实验表明,胶原腱组织中的不均匀折射率产生近场效果,即使大多数单独的原纤维效果可以忽略于20%-25%,也可以改变F / B比。 在组织中。 这是由阶段匹配条件上的后信号的灵敏度引起的。 (c)2018年光学学会

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